NAMPT Antibody | MyBioSource MBS9406172 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NAMPT Antibody

catalog :

MBS9406172

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using NAMPT antibody.

image 2 :

Immunohistochemical analysis of paraffin-embedded human ovary cancer using NAMPT antibody.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat spleen using NAMPT antibody at dilution of 1:200 (200x lens).

product information

catalog number :

MBS9406172

products type :

Antibody

products full name :

NAMPT Antibody

products short name :

[NAMPT]

products name syn :

[NAMPT; 1110035O14Rik; DKFZp666B131; MGC117256; PBEF]

other names :

[nicotinamide phosphoribosyltransferase; Nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin]

products gene name :

[NAMPT]

other gene names :

[NAMPT; NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik; PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor]

uniprot entry name :

NAMPT_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

491

specificity :

The antibody detects endogenous level of total NAMPT protein.

purity :

Antibodies were purified by affinity purification using immunogen.

form :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20 degree C

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

Western blotting: 1:500 - 1:2000. Immunohistochemistry: 1:50 - 1:200. Immunofluorescence: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemical analysis of paraffin-embedded human liver cancer using NAMPT antibody at dilution of 1:200 (200x lens).

image5 heading :

Immunofluorescence (IF)

image5 description :

Immunofluorescence analysis of HeLa cell using NAMPT antibody.

other info1 :

Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human NAMPT.

other info2 :

Target Name: NAMPT

products categories :

Total protein Ab

products description :

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.

ncbi gi num :

5031977

ncbi acc num :

NP_005737.1

ncbi gb acc num :

NM_005746.2

uniprot acc num :

P43490

ncbi mol weight :

52KD by SDS-PAGE

ncbi pathways :

Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (1269872); Circadian Clock Pathway (1269871); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Vitamins And Cofactors Pathway (1270144); Metabolism Of Water-soluble Vitamins And Cofactors Pathway (1270145); NAD Salvage Pathway (142214); Nicotinamide Salvaging Pathway (1270155); Nicotinate And Nicotinamide Metabolism Pathway (83014)

ncbi summary :

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

uniprot summary :

PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis. Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cell cycle regulation; EC 2.4.2.12; Transferase; Cytokine. Chromosomal Location of Human Ortholog: 7q22.3. Cellular Component: extracellular space; cytosol; nucleus. Molecular Function: protein binding; protein homodimerization activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; cytokine activity; drug binding; nicotinamide phosphoribosyltransferase activity. Biological Process: circadian rhythm; vitamin metabolic process; positive regulation of smooth muscle cell proliferation; female pregnancy; signal transduction; response to organic cyclic substance; NAD biosynthetic process; cell-cell signaling; nicotinamide metabolic process; NAD metabolic process; positive regulation of cell proliferation; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; water-soluble vitamin metabolic process; positive regulation of nitric-oxide synthase biosynthetic process

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage