Histone H3K27me3 Antibody | MyBioSource MBS9406121 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Histone H3K27me3 Antibody

catalog :

MBS9406121

quantity :

0.1 mL

price :

320 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E Coli., using H3K27me3antibody.

image 2 :

Dot-blot analysis of all sorts of methylation peptides using H3K27me3 antibody.

image 3 :

Immunofluorescence analysis of 293T cell using H3K27me3 antibody. Blue: DAPI for nuclear staining.

product information

catalog number :

MBS9406121

products type :

Antibody

products full name :

Histone H3K27me3 Antibody

products short name :

[Histone H3K27me3]

products name syn :

[HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3]

other names :

[histone H3.1t; Histone H3.1t; histone H3.1t; histone cluster 3, H3; H3/g]

other gene names :

[HIST3H3; HIST3H3; H3t; H3.4; H3/g; H3FT; H3FT; H3/t; H3t]

uniprot entry name :

H31T_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

136

purity :

Antibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.

form :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

concentration :

1.0 mg/ml

storage stability :

Store at -20°C or -80°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (CHIP)

app notes :

WB 1:500 - 1:2000. IHC 1:50-1:200. IF 1:50-1:200. IP 1:50-1:200. ChIP 1:50-1:200

image1 heading :

Western Blot (WB)

image2 heading :

Dot Blot (DB)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen Type: Peptide. Immunogen Description: A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3. SDS-PAGE MW: 15kDa

other info2 :

Modification: K27me3. Target Name: Histone H3

products categories :

Methyl-specific Ab

products description :

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

ncbi gi num :

4504299

ncbi acc num :

NP_003484.1

ncbi gb acc num :

NM_003493.2

uniprot acc num :

Q16695

ncbi mol weight :

15,508 Da

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Cell Cycle Pathway (1269741); Cell Cycle Checkpoints Pathway (1269742); Cell Cycle, Mitotic Pathway (1269763); Cellular Senescence Pathway (1270426); Cellular Responses To Stress Pathway (1270414); Chromosome Maintenance Pathway (1269853); Condensation Of Prophase Chromosomes Pathway (1269813); DNA Damage/Telomere Stress Induced Senescence Pathway (1270429)

ncbi summary :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

uniprot summary :

HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 1q42. Cellular Component: nucleoplasm; nucleus. Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity. Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance

size1 :

0.1 mL

price1 :

320 USD

more info or order :

MyBioSource product webpage