Human Vesicle-associated membrane protein 2, VAMP2 ELISA Kit | MyBioSource MBS940590 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Vesicle-associated membrane protein 2, VAMP2 ELISA Kit

catalog :

MBS940590

quantity :

48-Strip-Wells

price :

510 USD

product information

catalog number :

MBS940590

products type :

ELISA Kit

products full name :

Human Vesicle-associated membrane protein 2, VAMP2 ELISA Kit

products short name :

vesicle-associated membrane protein 2 (synaptobrevin 2)

products name syn :

Human Vesicle-associated membrane protein 2 (VAMP2) ELISA kit; FLJ11460; SYB2; VAMP-2; synaptobrevin 2; vesicle associated membrane protein 2; vesicle-associated membrane protein 2 (synaptobrevin 2)

other names :

vesicle-associated membrane protein 2; Vesicle-associated membrane protein 2; vesicle-associated membrane protein 2; VAMP-2; synaptobrevin-2; vesicle-associated membrane protein 2 (synaptobrevin 2); Synaptobrevin-2

products gene name :

VAMP2

other gene names :

VAMP2; VAMP2; SYB2; VAMP-2

uniprot entry name :

VAMP2_BOVIN

reactivity :

Human

sequence length :

116

specificity :

This assay has high sensitivity and excellent specificity for detection of human VAMP2. No significant cross-reactivity or interference between human VAMP2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml -1200 pg/ml. Sensitivity: The minimum detectable dose of human VAMP2 is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VAMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VAMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VAMP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VAMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

27806183

ncbi acc num :

NP_776908.1

ncbi gb acc num :

NM_174483.2

uniprot acc num :

P63026

ncbi mol weight :

12,649 Da

ncbi pathways :

Acetylcholine Neurotransmitter Release Cycle Pathway (582057); Clathrin Derived Vesicle Budding Pathway (581820); Glutamate Neurotransmitter Release Cycle Pathway (582055); Integration Of Energy Metabolism Pathway (581703); Membrane Trafficking Pathway (581816); Metabolism Pathway (581638); Neuronal System Pathway (582047); Neurotransmitter Release Cycle Pathway (582052); Regulation Of Insulin Secretion Pathway (581704); SNARE Interactions In Vesicular Transport Pathway (84296)

uniprot summary :

VAMP2: a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. Protein type: Motility/polarity/chemotaxis; Vesicle; Membrane protein, integral. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: zymogen granule membrane; neuron projection; intracellular membrane-bound organelle; synaptic vesicle membrane; clathrin-coated vesicle; secretory granule membrane; integral to plasma membrane; trans-Golgi network; cytosol; secretory granule; SNARE complex; synaptic vesicle; membrane; perinuclear region of cytoplasm; plasma membrane; synapse; cytoplasmic vesicle; cell junction. Molecular Function: calmodulin binding; SNAP receptor activity; protein binding; SNARE binding; protein self-association; syntaxin binding; syntaxin-1 binding; phospholipid binding; calcium-dependent protein binding. Biological Process: exocytosis; natural killer cell degranulation; neurotransmitter secretion; neutrophil degranulation; pathogenesis; calcium ion-dependent exocytosis; vesicle-mediated transport; vesicle fusion; regulation of exocytosis; synaptic transmission; protein transport; synaptic vesicle exocytosis; cellular protein metabolic process; glutamate secretion; cellular response to insulin stimulus; Golgi to plasma membrane protein transport; response to glucose stimulus; energy reserve metabolic process; eosinophil degranulation; protein complex assembly; post-Golgi vesicle-mediated transport; regulation of insulin secretion

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800