ELISA/assay

Human low-density lipoprotein-receptor-related protein 4 (LRP-4) antibody, IgG ELISA Kit

MBS940575

48-Strip-Wells

510 USD

ELISA Kit

Human low-density lipoprotein-receptor-related protein 4 (LRP-4) antibody, IgG ELISA Kit

low-density lipoprotein-receptor-related protein 4 (LRP-4) antibody (IgG)

Human low-density lipoprotein-receptor-related protein 4 (LRP-4) antibody (IgG) ELISA Kit; low-density lipoprotein-receptor-related protein 4 (LRP-4) antibody (IgG)

low-density lipoprotein receptor-related protein 4; Low-density lipoprotein receptor-related protein 4; low-density lipoprotein receptor-related protein 4; multiple epidermal growth factor-like domains 7; low density lipoprotein receptor-related protein 4; Multiple epidermal growth factor-like domains 7

LRP4; LRP4; CLSS; LRP-4; LRP10; MEGF7; SOST2; KIAA0816; LRP10; MEGF7; LRP-4

LRP4_HUMAN

Human

1,905

This assay has high sensitivity and excellent specificity for detection of human LRP-4 antibody (IgG). No significant cross-reactivity or interference between human LRP-4 antibody (IgG) and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human LRP-4 antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured.

157384998

NP_002325.2

NM_002334.3

O75096

212,045 Da

ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262)

This gene encodes a member of the low-density lipoprotein receptor-related protein family. The encoded protein may be a regulator of Wnt signaling. Mutations in this gene are associated with Cenani-Lenz syndrome. [provided by RefSeq, May 2010]

LRP4: Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN- induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Defects in LRP4 are the cause of Cenani-Lenz syndactyly syndrome (CLSS). It is a congenital malformation syndrome defined as complete and complex syndactyly of the hands combined with malformations of the forearm bones and similar manifestations in the lower limbs. Defects in LRP4 are the cause of sclerosteosis type 2 (SOST2). A sclerosing bone dysplasia characterized by a generalized hyperostosis and sclerosis leading to a markedly thickened skull, with mandible, ribs, clavicles and all long bones also being affected. Due to narrowing of the foramina of the cranial nerves, facial nerve palsy, hearing loss and atrophy of the optic nerves can occur. Sclerosteosis is clinically and radiologically very similar to van Buchem disease, mainly differentiated by hand malformations and a large stature in sclerosteosis patients. Belongs to the LDLR family. Protein type: Receptor, misc.; Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: cell surface; cell soma; dendrite; postsynaptic density; integral to membrane; flotillin complex; neuromuscular junction. Molecular Function: protein binding; protein homodimerization activity; apolipoprotein binding; calcium ion binding; receptor tyrosine kinase binding. Biological Process: limb development; extracellular matrix organization and biogenesis; regulation of protein amino acid phosphorylation; Wnt receptor signaling pathway; dendrite morphogenesis; endocytosis; odontogenesis of dentine-containing teeth; dorsal/ventral pattern formation; synaptic growth at neuromuscular junction; hair follicle development; negative regulation of ossification; synapse organization and biogenesis; protein heterotetramerization; negative regulation of axonogenesis; embryonic digit morphogenesis; kidney development; proximal/distal pattern formation. Disease: Myasthenic Syndrome, Congenital, 17; Cenani-lenz Syndactyly Syndrome; Sclerosteosis 2

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800