nNOS (Ab-852) Antibody | MyBioSource MBS9405740 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

nNOS (Ab-852) Antibody

catalog :

MBS9405740

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from Rat and Mouse brain tissue using nNOS(Ab-852) Antibody.

image 2 :

Immunofluorescence staining of methanol-fixed Hela cells using nNOS(Ab-852) Antibody.

product information

catalog number :

MBS9405740

products type :

Antibody

products full name :

nNOS (Ab-852) Antibody

products short name :

[nNOS]

products name syn :

[BNOS; Constitutive NOS; N-NOS; NC-NOS; NOS1]

other names :

[nitric oxide synthase, brain isoform 1; Nitric oxide synthase, brain; nitric oxide synthase, brain; nitric oxide synthase 1 (neuronal); Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS]

products gene name :

[nNOS]

other gene names :

[NOS1; NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS; N-NOS; nNOS]

uniprot entry name :

NOS1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

1434

specificity :

The antibody detects endogenous level of total nNOS protein.

purity :

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

form :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

tested application :

Western Blot (WB), Immunofluorescence (IF)

app notes :

Western blotting: 1:500~1:1000. Immunofluorescence: 1:100~1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen Type: Peptide-KLH. Immunogen Description: Peptide sequence around aa.850~854 (F-N-S-V-S) derived from Human nNOS.

other info2 :

Target Name: nNOS

products categories :

Total protein Ab

products description :

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter.

ncbi gi num :

10835173

ncbi acc num :

NP_000611.1

ncbi gb acc num :

NM_000620.4

uniprot acc num :

P29475

ncbi mol weight :

160kd

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Arginine Biosynthesis Pathway (82943); Arginine Biosynthesis Pathway (306)

ncbi summary :

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

uniprot summary :

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described. Protein type: Oxidoreductase; EC 1.14.13.39; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 12q24.22. Cellular Component: protein complex; cytoskeleton; photoreceptor inner segment; sarcoplasmic reticulum; mitochondrion; perinuclear region of cytoplasm; cytoplasm; dendritic spine; synapse; cytosol; sarcolemma; lipid raft. Molecular Function: calmodulin binding; protein binding; sodium channel regulator activity; FAD binding; NADPH-hemoprotein reductase activity; FMN binding; nitric-oxide synthase activity; iron ion binding; heme binding; oxidoreductase activity; NADP binding; cadmium ion binding. Biological Process: negative regulation of serotonin uptake; interaction with host; negative regulation of calcium ion transport; striated muscle contraction; negative regulation of potassium ion transport; positive regulation of transcription, DNA-dependent; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; positive regulation of vasodilation; response to heat; negative regulation of hydrolase activity; exogenous drug catabolic process; peptidyl-cysteine S-nitrosylation; response to hypoxia; arginine catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; negative regulation of blood pressure; blood coagulation; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; multicellular organismal response to stress; myoblast fusion; neurotransmitter biosynthetic process. Disease: Pyloric Stenosis, Infantile Hypertrophic, 1

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

255

more info or order :

MyBioSource product webpage