antibody

ASC Antibody

MBS9404874

0.05 mL

200 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry

Antibody

ASC Antibody

[ASC]

[Apoptosis-associated speck-like protein containing a CARD; hASC; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1; Caspase recruitment domain-containing protein 5]

[apoptosis-associated speck-like protein containing a CARD isoform a; Apoptosis-associated speck-like protein containing a CARD; apoptosis-associated speck-like protein containing a CARD; PYD and CARD domain containing; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1]

[ASC]

[PYCARD; PYCARD; ASC; TMS; TMS1; CARD5; TMS-1; ASC; CARD5; TMS1; hASC]

ASC_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat

195

The antibody detects endogenous levels of total ASC protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml

Store at -20 °C

Western Blot (WB), Immunohistochemistry (IHC)

Western blotting: 1:500~1:3000. Immunohistochemistry: 1:50~1:100

Western Blot (WB)

Immunohistochemistry (IHC)

Source: Human plasma. Immunogen Type: Peptide. Immunogen Description: Synthesized peptide derived from internal of human ASC. SDS-PAGE MW: 21 kDa

Target Name: ASC

Total protein Ab

Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the DAPIN and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern recognition receptors, such as NLRP2, NLRP3, AIM2 and probably IFI16. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered proinflammatory responses and inflammasome activation. Isoform 2 may have a regulating effect on the function as inflammasome adapter. Isoform 3 seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing.Masumoto J.,, J. Biol. Chem. 274:33835-33838(1999).Conway K.E., Cancer Res. 60:6236-6242(2000).McConnell B.B., Cancer Res. 60:6243-6247(2000)

10835256

NP_037390.2

NM_013258.4

Q9ULZ3

~54,000

C-type Lectin Receptors (CLRs) Pathway (1269303); CLEC7A (Dectin-1) Signaling Pathway (1269304); CLEC7A/inflammasome Pathway (1269307); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Direct P53 Effectors Pathway (137939); Immune System Pathway (1269170); Inflammasomes Pathway (1269253); Influenza A Pathway (217173); Influenza A Pathway (217150)

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt s lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold; Tumor suppressor. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: cell soma; mitochondrion; endoplasmic reticulum; cytoplasm; nucleolus; extracellular region; IkappaB kinase complex; cytosol; nucleus. Molecular Function: protein binding; protein homodimerization activity; protease binding; caspase activator activity; Pyrin domain binding. Biological Process: apoptosis; positive regulation of apoptosis; myeloid dendritic cell activation during immune response; regulation of protein stability; positive regulation of caspase activity; positive regulation of JNK cascade; positive regulation of interleukin-1 beta secretion; signal transduction; positive regulation of activated T cell proliferation; defense response to Gram-negative bacterium; activation of NF-kappaB transcription factor; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; inflammatory response; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; positive regulation of adaptive immune response; caspase activation; interleukin-1 beta production; positive regulation of interleukin-6 production; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of tumor necrosis factor production; negative regulation of interferon-beta production; myeloid dendritic cell activation; positive regulation of interferon-gamma production; positive regulation of actin filament polymerization; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; innate immune response; positive regulation of transcription factor activity; regulation of autophagy; positive regulation of T cell activation; positive regulation of defense response to virus by host; activation of innate immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II

0.05 mL

200 USD

0.1 mL

255