product summary
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company name :
MyBioSource
product type :
antibody
product name :
PPP1CA Antibody
catalog :
MBS9404690
quantity :
0.1 mL
price :
255 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of extracts of various cell lines, using PPP1CA antibody.
image 2 :
Immunohistochemical analysis of paraffin-embedded human kidney using PPP1CA antibody at dilution of 1:200 (400x lens).
image 3 :
Immunohistochemical analysis of paraffin-embedded human stomach cancer using PPP1CA antibody at dilution of 1:200 (400x lens).
product information
catalog number :
MBS9404690
products type :
Antibody
products full name :
PPP1CA Antibody
products short name :
[PPP1CA]
products name syn :
[PPP1CA; MGC15877; MGC1674; PP-1A; PP1alpha]
other names :
[serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 3; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; serine/threonine-protein phosphatase PP1-alpha catalytic subunit; protein phosphatase 1, catalytic subunit, alpha isozyme]
products gene name :
[PPP1CA]
other gene names :
[PPP1CA; PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha; PPP1A; PP-1A]
uniprot entry name :
PP1A_HUMAN
clonality :
Polyclonal
host :
Rabbit
reactivity :
Human, Mouse, Rat
sequence length :
341
specificity :
The antibody detects endogenous level of total PPP1CA protein.
purity :
Antibodies were purified by affinity purification using immunogen.
form :
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
concentration :
1.0 mg/ml
storage stability :
Store at -20°C
tested application :
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
app notes :
Western blotting: 1:500 - 1:2000. Immunohistochemistry: 1:50 - 1:100. Immunofluorescence: 1:50 - 1:200
image1 heading :
Western blot
image2 heading :
Immunohistochemical
image3 heading :
Immunohistochemical
image4 heading :
Immunofluorescence
image4 description :
Immunofluorescence analysis of U2OS cell using PPP1CA antibody. Blue: DAPI for nuclear staining.
other info1 :
Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human PPP1CA. SDS-PAGE MW: 38 kD
other info2 :
Target Name: PPP1CA
products categories :
Total protein Ab
products description :
Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1a, PP1d, PP1g1 and PP1g2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1a by cdc2 kinase inhibits PP1a activity (4).
ncbi gi num :
56790945
ncbi acc num :
NP_001008709.1
ncbi gb acc num :
NM_001008709.1
uniprot acc num :
P62136
ncbi pathways :
ALK1 Signaling Events Pathway (137968); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); BMP Receptor Signaling Pathway (137948); Circadian Clock Pathway (1269871); DARPP-32 Events Pathway (1269591)
ncbi summary :
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
uniprot summary :
PPP1CA: is a catalytic subunit of protein phosphatase 1 (PP1), aubiquitous cytosolic serine-threonine phosphatase. Composed of a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer s disease. Down-regulated in lung squamous cell carcinoma. Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor); Nucleolus. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: nucleoplasm; glycogen granule; cytoplasm; dendritic spine; plasma membrane; nucleolus; perikaryon; protein phosphatase type 1 complex; nucleus; cytosol. Molecular Function: ribonucleoprotein binding; protein binding; metal ion binding; phosphoric monoester hydrolase activity; protein serine/threonine phosphatase activity. Biological Process: glycogen metabolic process; regulation of glycogen biosynthetic process; cell cycle; protein amino acid dephosphorylation; regulation of circadian rhythm; entrainment of circadian clock by photoperiod; regulation of glycogen catabolic process; branching morphogenesis of a tube; cell division; transforming growth factor beta receptor signaling pathway; triacylglycerol catabolic process; circadian regulation of gene expression; negative regulation of transforming growth factor beta receptor signaling pathway; lung development
size1 :
0.1 mL
price1 :
255 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
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