PRKD1 Antibody | MyBioSource MBS9404610 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PRKD1 Antibody

catalog :

MBS9404610

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Gel: 6%SDS-PAGE Lysates (from left to right): 293T and NIH/3T3 cell Amount of lysate: 40ug per lane Primary antibody: 1/550 dilution Secondary antibody dilution: 1/8000 Exposure time: 30 seconds

image 2 :

Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using at dilution 1/40.

product information

catalog number :

MBS9404610

products type :

Antibody

products full name :

PRKD1 Antibody

products short name :

[PRKD1]

products name syn :

[PKD; PKCM; PRKCM; PKC-MU]

other names :

[serine/threonine-protein kinase D1; Serine/threonine-protein kinase D1; serine/threonine-protein kinase D1; protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu]

products gene name :

[PRKD1]

other gene names :

[PRKD1; PRKD1; PKD; PKCM; PRKCM; PKC-MU; PKD; PKD1; PRKCM]

uniprot entry name :

KPCD1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

912

specificity :

The antibody detects endogenous levels of total PRKD1 protein.

purity :

Antigen affinity purification.

form :

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

concentration :

3.3 mg/ml

storage stability :

Store at -20 degree C

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Western blotting: 1:500-1:2000. Immunohistochemistry: 1:50-1:200

image1 heading :

Testing Data

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen Type: Peptide. Immunogen Description: Synthetic peptide corresponding to residues near the C terminal of human protein kinase D1

other info2 :

Target Name: PRKD1

products categories :

Total protein Ab

products description :

PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion.

ncbi gi num :

115529463

ncbi acc num :

NP_002733

ncbi gb acc num :

NM_002742.2

uniprot acc num :

Q15139

ncbi mol weight :

102kd

ncbi pathways :

B Cell Receptor Signaling Pathway (198909); Calcium Regulation In The Cardiac Cell Pathway (198906); EGFR1 Signaling Pathway (198782); G Protein Signaling Pathways (198849); IGF1 Pathway (137992); LPA Receptor Mediated Events Pathway (137928); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Myometrial Relaxation And Contraction Pathways (198759); Rap1 Signaling Pathway (868086)

ncbi summary :

uniprot summary :

PRKD1: a CAMK kinase of the PKD family. Cleavage by caspase-3 following DNA damage activates it and alters its subcellular localization. Sensitizes cells to apoptosis induced by genotoxic stress. Its cleavage is blocked in cells that over-express the anti-apoptotic Bcl-x(L) protein. Expression of a caspase-resistant mutant partially inhibits DNA damage-induced apoptosis. Its activation by TLR ligands is dependent on MyD88, IRAK4 and -1, but not TRAF6. Essential for MyD88-dependent proinflammatory immune responses. Activated by diacylglycerol and phorbol esters. Binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface. Colocalizes with F-actin at peripheral F-actin-rich structures in membrane ruffles at the edge of lamellipodia in cervical carcinoma cells. Substrates reportedly include critical regulatory proteins including CREB, SSH1L, CTNNB1, HDACs 5 and 7, PKD1, HPK1, MARK2, PIP5K2A, PPP1R14A. Protein type: Protein kinase, CAMK; Motility/polarity/chemotaxis; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Autophagy; CAMK group; PKD family. Chromosomal Location of Human Ortholog: 14q11. Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; intercellular junction; cell cortex; trans-Golgi network; nucleus; cytosol. Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ATP binding. Biological Process: integrin-mediated signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; sphingolipid metabolic process; sphingolipid biosynthetic process; apoptosis; negative regulation of endocytosis; protein amino acid autophosphorylation; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; signal transduction; positive regulation of peptidyl-serine phosphorylation; Golgi vesicle transport; activation of NF-kappaB transcription factor; cell proliferation; positive regulation of osteoblast differentiation; positive regulation of angiogenesis; peptidyl-serine phosphorylation; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; vascular endothelial growth factor receptor signaling pathway; inflammatory response; Golgi organization and biogenesis

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage