catalog number :
MBS940453
products type :
ELISA Kit
products full name :
Human casein alpha s1, CSN1S1 ELISA Kit
products short name :
casein alpha s1
products name syn :
Human casein alpha s1 (CSN1S1) ELISA kit; CASA; CSN1; MGC149368; casein; alpha; casein alpha s1
other names :
alpha-S1-casein isoform 2; Alpha-S1-casein; alpha-S1-casein; casein, alpha; casein alpha s1
products gene name :
CSN1S1
other gene names :
CSN1S1; CSN1S1; CASA; CSN1; CASA; CSN1
uniprot entry name :
CASA1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human CSN1S1. No significant cross-reactivity or interference between human CSN1S1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Breast milk. Assay Type: Competitive. Detection Range: 37.5 ?g/ml-600 ?g/ml. Sensitivity: The minimum detectable dose of human CSN1S1 is typically less than 18.75? g/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human CSN1S1 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for CSN1S1 and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated CSN1S1 and CSN1S1 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of CSN1S1 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001020275.1
ncbi gb acc num :
NM_001025104.1
ncbi mol weight :
21,671 Da
uniprot summary :
CSN1S1: Important role in the capacity of milk to transport calcium phosphate. Heteromultimers of alpha-s1 casein and kappa-casein; disulfide-linked. Mammary gland specific. Secreted in milk. Belongs to the alpha-casein family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q21.1. Cellular Component: extracellular space; extracellular region. Molecular Function: transporter activity. Biological Process: transport
size4 :
10x96-Strip-Wells