SIRT3 antibody | MyBioSource MBS9403926 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

SIRT3 antibody

catalog :

MBS9403926

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Immunofluorescence analysis of MCF7 cell using SIRT3 antibody. Blue: DAPI for nuclear staining.

image 2 :

Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody at dilution of 1:100 (400x lens).

product information

catalog number :

MBS9403926

products type :

Antibody

products full name :

SIRT3 antibody

products short name :

[SIRT3]

products name syn :

[SIR2L3]

other names :

[NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform b; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; sirtuin 3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3]

products gene name :

[SIRT3]

other gene names :

[SIRT3; SIRT3; SIR2L3; SIR2L3; hSIRT3]

uniprot entry name :

SIR3_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

257

specificity :

The antibody detects endogenous level of total SIRT3 antibody.

purity :

Antibodies were purified by affinity purification using immunogen.

form :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20°C

tested application :

Western Blot (WB), Immunofluorescence (IF)

app notes :

Western blotting: 1:500 - 1:2000. Immunofluorescence: 1:50 - 1:100

image1 heading :

Immunofluorescence (IF)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen Type: Peptide. Immunogen Description: A synthetic peptide of human SIRT3. SDS-PAGE MW: 44 kd

other info2 :

Target Name: SIRT3

products categories :

Total protein Ab

products description :

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.

ncbi gi num :

63054862

ncbi acc num :

NP_001017524.1

ncbi gb acc num :

NM_001017524.2

uniprot acc num :

Q9NTG7

ncbi mol weight :

44kd

ncbi pathways :

Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Energy Metabolism Pathway (198907); Mitochondrial Biogenesis Pathway (1268839); Organelle Biogenesis And Maintenance Pathway (1268838); Signaling Events Mediated By HDAC Class I Pathway (137997); Signaling Events Mediated By HDAC Class III Pathway (137972); Transcriptional Activation Of Mitochondrial Biogenesis Pathway (1268841)

ncbi summary :

uniprot summary :

SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product. Protein type: Mitochondrial; Deacetylase; EC 3.5.1.-. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: mitochondrion; membrane; mitochondrial matrix. Molecular Function: protein binding; enzyme binding; zinc ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD+ ADP-ribosyltransferase activity. Biological Process: mitochondrion organization and biogenesis; protein amino acid ADP-ribosylation; protein amino acid deacetylation; organelle organization and biogenesis; aerobic respiration; aging

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage