catalog number :
MBS940392
products type :
ELISA Kit
products full name :
Mouse Bone morphogenetic protein 5, BMP5 ELISA Kit
products short name :
bone morphogenetic protein 5
products name syn :
Mouse Bone morphogenetic protein 5 (BMP5) ELISA kit; MGC34244; ; bone morphogenetic protein 5
other names :
bone morphogenetic protein 5; Bone morphogenetic protein 5; bone morphogenetic protein 5; BMP-5; short ear; bone morphogenetic protein 5
products gene name :
BMP5
other gene names :
Bmp5; Bmp5; se; AU023399; Bmp-5; BMP-5
uniprot entry name :
BMP5_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse BMP5. No significant cross-reactivity or interference between mouse BMP5 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of mouse BMP5 is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_031581.2
ncbi gb acc num :
NM_007555.3
ncbi mol weight :
51,512 Da
ncbi pathways :
Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474); TGF-beta Signaling Pathway (83261); TGF-beta Signaling Pathway (475)
uniprot summary :
BMP5: Induces cartilage and bone formation. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: ossification; multicellular organismal development; negative regulation of insulin-like growth factor receptor signaling pathway; pattern specification process; regulation of apoptosis; BMP signaling pathway; negative regulation of cell proliferation; regulation of MAPKKK cascade; cartilage development; negative regulation of aldosterone biosynthetic process; male genitalia development; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; skeletal development; cell development; positive regulation of epithelial cell proliferation; growth
size4 :
10x96-Strip-Wells