DLD Antibody | MyBioSource MBS9403435 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

DLD Antibody

catalog :

MBS9403435

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using DLD antibody.

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast cancer using DLD antibody at dilution of 1:200 (200x lens).

image 3 :

Immunohistochemical analysis of paraffin-embedded human esophageal cancer using DLD antibody at dilution of 1:200 (200x lens).

product information

catalog number :

MBS9403435

products type :

Antibody

products full name :

DLD Antibody

products short name :

[DLD]

products name syn :

[E3; LAD; DLDD; DLDH; GCSL]

other names :

[dihydrolipoyl dehydrogenase, mitochondrial isoform 1; Dihydrolipoyl dehydrogenase, mitochondrial; dihydrolipoyl dehydrogenase, mitochondrial; dihydrolipoamide dehydrogenase; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein]

products gene name :

[DLD]

other gene names :

[DLD; DLD; E3; LAD; DLDD; DLDH; GCSL; PHE3; GCSL; LAD; PHE3]

uniprot entry name :

DLDH_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

509

specificity :

The antibody detects endogenous level of total DLD protein.

purity :

Antibodies were purified by affinity purification using immunogen.

form :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20°C

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

Western blotting: 1:500 - 1:2000. Immunohistochemistry: 1:50 - 1:200. Immunofluorescence: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemical analysis of paraffin-embedded human stomach using DLD antibody at dilution of 1:200 (200x lens).

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Immunohistochemical analysis of paraffin-embedded human stomach using DLD antibody at dilution of 1:200 (400x lens).

other info1 :

Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human DLD. SDS-PAGE MW: 54kD

other info2 :

Target Name: DLD

products categories :

Total protein Ab

products description :

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

ncbi gi num :

91199540

ncbi acc num :

NP_000099.2

ncbi gb acc num :

NM_000108.4

uniprot acc num :

P09622

ncbi pathways :

2-oxobutanoate Degradation Pathway (142413); 2-oxobutanoate Degradation I Pathway (139464); 2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway (142336); 2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway (139119); 2-oxoisovalerate Decarboxylation To Isobutanoyl-CoA Pathway (142335); 2-oxoisovalerate Decarboxylation To Isobutanoyl-CoA Pathway (139118); Biosynthesis Of Antibiotics Pathway (1144997); Branched-chain Amino Acid Catabolism Pathway (1270161); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567)

ncbi summary :

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

uniprot summary :

DLD: a multi-functional mitochondrial enzyme. An enzymatic component of the mitochondrial glycine cleavage system, the pyruvate dehydrogenase complex (PDHC), the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Is the E3 component of the PDHC that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. The E3 component has dihydrolipoamide dehydrogenase activity. The PDHC contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. Defects in DLD are a cause of maple syrup urine disease (MSUD), characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation. Differentially expressed in the Wernicke s Area from patients with schizophrenia. Inhibited by 5-methoxyindole-2-carboxylic acid (MICA). Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - citrate (TCA) cycle; Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Oxidoreductase; Mitochondrial; EC 1.8.1.4; Carbohydrate Metabolism - pyruvate. Chromosomal Location of Human Ortholog: 7q31-q32. Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; acrosomal matrix; cilium. Molecular Function: mercury (II) reductase activity; FAD binding; mercury ion binding; dihydrolipoyl dehydrogenase activity; NADP binding. Biological Process: mitochondrial electron transport, NADH to ubiquinone; tricarboxylic acid cycle; gastrulation; regulation of acetyl-CoA biosynthetic process from pyruvate; branched chain family amino acid catabolic process; proteolysis; cellular metabolic process; regulation of membrane potential; cell redox homeostasis; detoxification of mercury ion; lysine catabolic process; pyruvate metabolic process; sperm capacitation. Disease: Dihydrolipoamide Dehydrogenase Deficiency

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage