ELISA/assay

Human Sortilin, SORT1 ELISA Kit

MBS940316

48-Strip-Wells

510 USD

ELISA Kit

Human Sortilin, SORT1 ELISA Kit

sortilin 1

Human Sortilin (SORT1) ELISA kit; Gp95; NT3; neurotensin receptor 3; sortilin 1

sortilin isoform 2; Sortilin; sortilin; NTR3; glycoprotein 95; 100 kDa NT receptor; neurotensin receptor 3; sortilin 1; 100 kDa NT receptor; Glycoprotein 95; Gp95; Neurotensin receptor 3; NT3; NTR3

SORT1

SORT1; SORT1; NT3; Gp95; LDLCQ6; Gp95; NT3; NTR3

SORT_HUMAN

Human

694

This assay has high sensitivity and excellent specificity for detection of Human SORT1. No significant cross-reactivity or interference between Human SORT1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml-3000 pg/ml. Sensitivity: 11.72 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Differentiation

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sortilin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sortilin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sortilin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sortilin bound in the initial step. The color development is stopped and the intensity of the color is measured.

327315343

NP_001192157.1

NM_001205228.1

Q99523

92,068 Da

Clathrin Derived Vesicle Budding Pathway (119545); Golgi Associated Vesicle Biogenesis Pathway (119546); Lysosome Pathway (99052); Lysosome Pathway (96865); Membrane Trafficking Pathway (106160); Neurotrophin Signaling Pathway (101143); Neurotrophin Signaling Pathway (100064); P75(NTR)-mediated Signaling Pathway (138019); Trans-Golgi Network Vesicle Budding Pathway (106165)

This gene encodes a protein that is a multi-ligand type-1 receptor with similarity to the yeast carboxypeptidase Y sorting receptor Vps10 protein. The encoded protein, a trans-Golgi network (TGN) transmembrane protein, binds a number of unrelated ligands that participate in a wide range of cellular processes; however, it lacks the typical features of a signalling receptor. In the TGN, furin mediates the activation of the mature binding form. The encoded protein consists of a large luminal domain, a single transmembrane segment and short C-terminal cytoplasmic tail. The luminal domain contains a cysteine-rich region similar to two corresponding segments in the yeast Vps10p; the cytoplasmic tail is similar to the corresponding segment of the cation-independent mannose 6-phosphate receptor and the tail also interacts with the VHS domains of GGA (Golgi-associated, gamma-adaptin homologous, ARF-interacting) proteins. [provided by RefSeq, Jul 2008]

SORT1: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. A common polymorphism located in a non-coding region between CELSR2 and PSRC1 alters a CEBP transcription factor binding site and is responsible for changes in hepatic expression of SORT1. Altered SORT1 expression in liver affects low density lipoprotein cholesterol levels in plasma and is associated with susceptibility to myocardial infarction. Belongs to the VPS10-related sortilin family. SORT1 subfamily. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 1p13.3 1p21.3-p13.1. Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; nuclear membrane; cell surface; clathrin-coated vesicle; lysosomal membrane; early endosome; cytoplasmic membrane-bound vesicle; integral to membrane; coated pit; trans-Golgi network transport vesicle; perinuclear region of cytoplasm; plasma membrane; endosome membrane. Molecular Function: protein binding; nerve growth factor receptor activity; enzyme binding; nerve growth factor binding; neurotensin receptor activity, non-G-protein coupled. Biological Process: ossification; nerve growth factor receptor signaling pathway; multicellular organismal development; endosome transport via multivesicular body sorting pathway; vesicle organization and biogenesis; Golgi to endosome transport; myotube differentiation; endocytosis; response to insulin stimulus; G-protein coupled receptor protein signaling pathway; induction of apoptosis via death domain receptors; regulation of gene expression; neuropeptide signaling pathway; glucose import; endosome to lysosome transport; plasma membrane to endosome transport; negative regulation of lipoprotein lipase activity. Disease: Low Density Lipoprotein Cholesterol Level Quantitative Trait Locus 6

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800