antibody

PERK (Phospho-Thr981) Polyclonal Antibody

MBS9402602

0.05 mL

200 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

PERK (Phospho-Thr981) Polyclonal Antibody

[PERK]

[EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK]

[eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1; Eukaryotic translation initiation factor 2-alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK]

[PERK]

[EIF2AK3; EIF2AK3; PEK; WRS; PERK; PEK; PERK; HsPEK]

E2AK3_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat

1116

Phospho-PERK (T981) Polyclonal Antibody detects endogenous levels of PERK protein only when phosphorylated at T981.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

1 mg/ml

Store at -20°C/1 year

Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)

Immunohistochemistry: 1:100 - 1:300. ELISA: 1:40000. Western Blot: 1:500-2000

Western Blot (WB)

Immunogen Type: peptide. Immunogen Description: Synthesized peptide derived from human PERK around the phosphorylation site of T981. SDS-PAGE MW: 130kD

Modification: Phospho-Thr981. Target Species: Human. Target Name: PERK

Phospho-specific Ab

134304838

NP_004827.4

NM_004836.6

Q9NZJ5

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Influenza A Pathway (217173)

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK. Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, Other; Translation; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; PEK family; PEK subfamily. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytoplasm; integral to endoplasmic reticulum membrane. Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; enzyme binding; protein phosphatase binding; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity. Biological Process: fat cell differentiation; lactation; positive regulation of protein binding; translation; protein amino acid autophosphorylation; protein amino acid phosphorylation; bone mineralization; positive regulation of transcription from RNA polymerase I promoter; cellular response to glucose starvation; regulation of fatty acid metabolic process; angiogenesis; skeletal development; protein homooligomerization; ER overload response; caspase activation; endoplasmic reticulum organization and biogenesis; ossification; negative regulation of myelination; unfolded protein response; calcium-mediated signaling; chondrocyte development; SREBP-mediated signaling pathway; endocrine pancreas development; peptidyl-serine phosphorylation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; insulin-like growth factor receptor signaling pathway; insulin secretion; negative regulation of translation; negative regulation of translation initiation in response to stress; negative regulation of apoptosis. Disease: Epiphyseal Dysplasia, Multiple, With Early-onset Diabetes Mellitus

0.05 mL

200 USD

0.1 mL

255