Human Semaphorin-4A, SEMA4A ELISA Kit | MyBioSource MBS940246 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Semaphorin-4A, SEMA4A ELISA Kit

catalog :

MBS940246

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS940246

products type :

ELISA Kit

products full name :

Human Semaphorin-4A, SEMA4A ELISA Kit

products short name :

sema domain, immunoglobulin domain (Ig) , transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A

products name syn :

Human Semaphorin-4A (SEMA4A) ELISA kit; RP11-54H19.2; CORD10; FLJ12287; RP35; SEMAB; SEMB; OTTHUMP00000015917; OTTHUMP00000015918; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; 4A; semaphori; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; (semaphorin) 4A

other names :

semaphorin-4A isoform 1; Semaphorin-4A; semaphorin-4A; sema B; semaphorin-B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; Semaphorin-B; Sema B

products gene name :

SEMA4A

other gene names :

SEMA4A; SEMA4A; RP35; SEMB; SEMAB; CORD10; SEMAB; SEMB; UNQ783/PRO1317; Sema B

uniprot entry name :

SEM4A_HUMAN

reactivity :

Human

sequence length :

761

specificity :

This assay has high sensitivity and excellent specificity for detection of human SEMA4A. No significant cross-reactivity or interference between human SEMA4A and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: The minimum detectable dose of human SEMA4A is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

300863072

ncbi acc num :

NP_001180229.1

ncbi gb acc num :

NM_001193300.1

uniprot acc num :

Q9H3S1

ncbi mol weight :

83,574 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Other Semaphorin Interactions Pathway (119526); Semaphorin Interactions Pathway (119519)

ncbi summary :

This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]

uniprot summary :

SEMA4A: a single-pass type I membrane protein that inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Defects in SEMA4A are the cause of retinitis pigmentosa type 35 and cone-rod dystrophy type 10. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q22. Cellular Component: plasma membrane; integral to membrane. Molecular Function: protein binding; receptor activity. Biological Process: axon guidance; regulation of cell shape; negative regulation of angiogenesis; T-helper 1 cell differentiation; angiogenesis. Disease: Retinitis Pigmentosa 35; Cone-rod Dystrophy 10

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage