ELISA/assay

Mouse Glutamate [NMDA] receptor subunit epsilon-1, GRIN2A ELISA Kit

MBS940236

48-Strip-Wells

510 USD

ELISA Kit

Mouse Glutamate [NMDA] receptor subunit epsilon-1, GRIN2A ELISA Kit

glutamate receptor, ionotropic, N-methyl D-aspartate 2A

Mouse Glutamate [NMDA] receptor subunit epsilon-1 (GRIN2A) ELISA kit; NMDAR2A; NR2A; N-methyl-D-aspartate receptor channel; subunit epsilon-1; N-methyl-D-aspartate receptor subunit 2A; NMDA receptor subtype 2A; OTTHUMP00000160135; OTTHUMP00000174531; glutamate receptor; ionotropic; N-methyl D-aspartate 2A

glutamate receptor ionotropic, NMDA 2A; Glutamate receptor ionotropic, NMDA 2A; glutamate receptor ionotropic, NMDA 2A; GluRepsilon1; N-methyl D-aspartate receptor subtype 2A; glutamate [NMDA] receptor subunit epsilon-1; glutamate receptor, ionotropic, NMDA2A (epsilon 1); Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A

GRIN2A

Grin2a; Grin2a; NR2A; GluN2A; NMDAR2A; GluN2A; NMDAR2A; NR2A

NMDE1_MOUSE

Mouse

1464

This assay has high sensitivity and excellent specificity for detection of Mouse GRIN2A. No significant cross-reactivity or interference between Mouse GRIN2A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 37.5 pg/ml-2400 pg/ml. Sensitivity: 9.3 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 2: Anion transport. Protein Biological Process 3: Ion transport

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GRIN2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GRIN2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRIN2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRIN2A bound in the initial step. The color development is stopped and the intensity of the color is measured.

41680705

NP_032196.2

NM_008170.2

P35436

165,421 Da

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (575080); Alcoholism Pathway (585577); Alcoholism Pathway (587116); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amphetamine Addiction Pathway (547634); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83296); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Calcium Signaling Pathway (83247)

NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated. Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; membrane; endoplasmic reticulum; postsynaptic density; dendritic spine; integral to membrane; plasma membrane; terminal button; synapse; cell junction; N-methyl-D-aspartate selective glutamate receptor complex. Molecular Function: voltage-gated cation channel activity; protein dimerization activity; neurotransmitter binding; glutamate receptor binding; zinc ion binding; metal ion binding; calcium channel activity; ion channel activity; receptor activity; protein kinase binding; ATPase binding; protein binding; glutamate binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; protein complex binding; cell adhesion molecule binding; cation channel activity; receptor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; startle response; positive regulation of apoptosis; rhythmic process; regulation of synaptic plasticity; locomotory behavior; sensory perception of pain; dopamine metabolic process; synaptic transmission; protein localization; learning and/or memory; transport; calcium ion transport; response to wounding; visual learning; locomotion; serotonin metabolic process; cation transport; protein tetramerization; response to drug; regulation of synaptic transmission; regulation of action potential; synaptic transmission, glutamatergic; response to amphetamine; learning; sleep; memory; detection of mechanical stimulus involved in sensory perception of pain; regulation of membrane potential; response to ethanol; regulation of postsynaptic membrane potential; neurogenesis; directional locomotion; ionotropic glutamate receptor signaling pathway; ion transport; regulation of sensory perception of pain; negative regulation of protein catabolic process; regulation of excitatory postsynaptic membrane potential

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800