BAF170 Polyclonal Antibody | MyBioSource MBS9401447 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

BAF170 Polyclonal Antibody

catalog :

MBS9401447

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of COLO205 cells using BAF170 Polyclonal Antibody

image 2 :

Western Blot analysis of 293T AD293 22RV1 HELA HepG2 cells using BAF170 Polyclonal Antibody

product information

catalog number :

MBS9401447

products type :

Antibody

products full name :

BAF170 Polyclonal Antibody

products short name :

[BAF170]

products name syn :

[SMARCC2; BAF170; SWI/SNF complex subunit SMARCC2; BRG1-associated factor 170; BAF170; SWI/SNF complex 170 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2]

other names :

[SWI/SNF complex subunit SMARCC2 isoform c; SWI/SNF complex subunit SMARCC2; SWI/SNF complex subunit SMARCC2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2; BRG1-associated factor 170; BAF170; SWI/SNF complex 170 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2]

products gene name :

[BAF170]

other gene names :

[SMARCC2; SMARCC2; Rsc8; BAF170; CRACC2; BAF170; BAF170]

uniprot entry name :

SMRC2_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

1152

specificity :

BAF170 Polyclonal Antibody detects endogenous levels of BAF170 protein.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

form :

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

concentration :

1 mg/ml

storage stability :

Store at -20°C/1 year

tested application :

Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)

app notes :

Western Blot: 1:500 - 1:2000. Immunofluorescence: 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

other info1 :

. SDS-PAGE MW: 160 kd. Immunogen Type: peptide. Immunogen Description: Synthesized peptide derived from the Internal region of human BAF170.

other info2 :

Target Name: BAF170

products categories :

Total protein Ab

ncbi gi num :

194363725

ncbi acc num :

NP_001123892.1

ncbi gb acc num :

NM_001130420.1

uniprot acc num :

Q8TAQ2

ncbi pathways :

Chromatin Modifying Enzymes Pathway (1270434); Chromatin Organization Pathway (1270433); Glucocorticoid Receptor Regulatory Network Pathway (138014); Prostate Cancer Pathway (755440); RMTs Methylate Histone Arginines Pathway (1270439); TNF-alpha/NF-kB Signaling Pathway (198884)

ncbi summary :

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

SMARCC2: a core component of the BAF (SWI/SNF) complex. This family of complexes remodels chromatin structures, enabling transcription factors to gain access to DNA. They play important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling is due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain BAF53A and PHF10, are exchanged for homologous alternative BAF53B and BAF45B or BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene. Component of 6 multiprotein chromatin-remodeling complexes: Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I), WINAC and Brg1(II). Each of the five complexes contains a catalytic subunit (either SMARCA4 or SMARCA2), and at least SMARCE1, BAF53A or BAF53B, SMARCC2 and SMARCB1. Other subunits specific to each of the complexes may also be present. Component of the BAF (hSWI/SNF) complex, which includes at least actin (ACTB), ARID1A, ARID1B, SMARCA2, SMARCA4, BAF53A, BAF53B, SMARCE1 SMARCC1, SMARCC2, SMARCB1, and one or more of SMARCD1, SMARCD2, or SMARCD3. In muscle cells, the BAF complex also contains DPF3. May also interact with the SIN3A histone deacetylase transcription repressor complex in conjunction with SMARCA2 and SMARCA4. The minimal complex composed of SMARCC1 and SMARCA4 seems to be able to associate with cyclin such as CCNE1 or transcription factors such as KLF1 or GATA1. Ubiquitously expressed. Belongs to the SMARCC family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription, coactivator/corepressor; DNA-binding. Chromosomal Location of Human Ortholog: 12q13.2. Cellular Component: nucleoplasm; SWI/SNF complex; protein complex; transcriptional repressor complex; nuclear chromatin. Molecular Function: protein binding; nucleosomal DNA binding; transcription coactivator activity. Biological Process: regulation of transcription from RNA polymerase II promoter; chromatin remodeling; establishment and/or maintenance of chromatin architecture; nucleosome disassembly; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter involved in forebrain neuron fate commitment; ATP-dependent chromatin remodeling; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

255

more info or order :

MyBioSource product webpage