HPRT1 Antibody | MyBioSource MBS9401129 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HPRT1 Antibody

catalog :

MBS9401129

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using HPRT1 antibody.

product information

catalog number :

MBS9401129

products type :

Antibody

products full name :

HPRT1 Antibody

products short name :

[HPRT1]

products name syn :

[HPRT; HGPRT]

other names :

[hypoxanthine-guanine phosphoribosyltransferase; Hypoxanthine-guanine phosphoribosyltransferase; hypoxanthine-guanine phosphoribosyltransferase; hypoxanthine phosphoribosyltransferase 1]

products gene name :

[HPRT1]

other gene names :

[HPRT1; HPRT1; HPRT; HGPRT; HPRT; HGPRT; HGPRTase]

uniprot entry name :

HPRT_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

218

specificity :

The antibody detects endogenous level of total HPRT1 protein.

purity :

Antibodies were purified by affinity purification using immunogen.

form :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20 degree C

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Western blotting: 1:500 - 1:2000. Immunohistochemistry: 1:50 - 1:200

image1 heading :

Western Blot (WB)

other info1 :

Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human HPRT1.

other info2 :

Target Name: HPRT1

products categories :

Total protein Ab

products description :

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

ncbi gi num :

4504483

ncbi acc num :

NP_000185.1

ncbi gb acc num :

NM_000194.2

uniprot acc num :

P00492

ncbi mol weight :

24KD

ncbi pathways :

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Nucleotides Pathway (1270133); Nucleotide Metabolism Pathway (198876); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (1270134); Purine Metabolism Pathway (307); Purine Salvage Pathway (1270136)

ncbi summary :

uniprot summary :

HPRT1: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS). LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation. Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT); also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia. Belongs to the purine/pyrimidine phosphoribosyltransferase family. Protein type: EC 2.4.2.8; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; Cell development/differentiation; Nucleotide Metabolism - purine. Chromosomal Location of Human Ortholog: Xq26.1. Cellular Component: cytoplasm; cytosol. Molecular Function: protein binding; protein homodimerization activity; nucleotide binding; magnesium ion binding; hypoxanthine phosphoribosyltransferase activity. Biological Process: grooming behavior; hypoxanthine metabolic process; lymphocyte proliferation; IMP salvage; striatum development; nucleobase, nucleoside and nucleotide metabolic process; GMP salvage; cytolysis; dendrite morphogenesis; response to amphetamine; adenine salvage; locomotory behavior; purine nucleotide biosynthetic process; dopamine metabolic process; purine base metabolic process; purine salvage; protein homotetramerization; cerebral cortex neuron differentiation; GMP catabolic process; positive regulation of dopamine metabolic process; IMP metabolic process; hypoxanthine salvage; purine ribonucleoside salvage; central nervous system neuron development; guanine salvage. Disease: Kelley-seegmiller Syndrome; Lesch-nyhan Syndrome

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage