KRT20 Antibody | MyBioSource MBS9400927 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

KRT20 Antibody

catalog :

MBS9400927

quantity :

0.1 mL

price :

255 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from Hela cell and mouse intestines tissue, using KRT20 antibody.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon cancer using KRT20 antibody.

image 3 :

Immunohistochemical analysis of paraffin-embedded human rectal cancer using KRT20 antibody at dilution of 1:200 (200x lens).

product information

catalog number :

MBS9400927

products type :

Antibody

products full name :

KRT20 Antibody

products short name :

[KRT20]

products name syn :

[KRT20; K20; Cytokeratin20; KRT21; CK20]

other names :

[keratin, type I cytoskeletal 20; Keratin, type I cytoskeletal 20; keratin, type I cytoskeletal 20; keratin 20, type I; Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT]

products gene name :

[KRT20]

other gene names :

[KRT20; KRT20; K20; CD20; CK20; CK-20; KRT21; CK-20; K20]

uniprot entry name :

K1C20_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

424

specificity :

The antibody detects endogenous level of total KRT20 protein.

purity :

Antibodies were purified by affinity purification using immunogen.

form :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20°C

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

Western blotting: 1:500 - 1:2000. Immunohistochemistry: 1:50 - 1:200. Immunofluorescence: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunofluorescence (IF)

image4 description :

Immunofluorescence analysis of HeLa cell using KRT20 antibody. Blue: DAPI for nuclear staining.

other info1 :

Immunogen Type: Recombinant Protein. Immunogen Description: Recombinant protein of human KRT20. SDS-PAGE MW: 48kD

other info2 :

Target Name: KRT20

products categories :

Total protein Ab

products description :

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

ncbi gi num :

27894337

ncbi acc num :

NP_061883.1

ncbi gb acc num :

NM_019010.2

uniprot acc num :

P35900

ncbi summary :

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

uniprot summary :

K20: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21.2. Cellular Component: cytoplasm; intermediate filament. Molecular Function: protein binding; structural constituent of cytoskeleton. Biological Process: regulation of protein secretion; apoptosis; cellular response to stress; intermediate filament organization

size1 :

0.1 mL

price1 :

255 USD

more info or order :

MyBioSource product webpage