c-Fos Monoclonal Antibody | MyBioSource MBS9400504 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

c-Fos Monoclonal Antibody

catalog :

MBS9400504

quantity :

0.05 mL

price :

200 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[7D6-A9-D4]

reactivity :

human, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot detection of c-Fos antibody in HCT116,COS7 and C6 cell lysates using c-Fos antibody (1ïš500 diluted).Predicted band size:62KDa.Observed band size:62KDa.

image 2 :

Western blot detection of c-Fos antibody in Hela(TSA treated) and Hela cell lysates using c-Fos antibody (1:500 diluted).Predicted band size:62KDa.Observed band size:62KDa.

image 3 :

Western blot analysis of extracts from Hela cells,untreated,50 uM TSA-treated or 50 uM TPA-treated for 24h,using C-FOS antibody (1:500 diluted)(upper) or GAPDH antibody (1:10000 diluted) (lower).

product information

catalog number :

MBS9400504

products type :

Antibody

products full name :

c-Fos Monoclonal Antibody

products short name :

[c-Fos]

products name syn :

[Activator protein 1; AP 1; Cellular oncogene c fos; Cellular oncogene fos; FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral v fos oncogene homolog; FBJ Osteosarcoma Virus; FOS; FOS protein; FOS_HUMAN]

other names :

[proto-oncogene c-Fos; Proto-oncogene c-Fos; proto-oncogene c-Fos; FBJ murine osteosarcoma viral oncogene homolog; Cellular oncogene fos; G0/G1 switch regulatory protein 7]

products gene name :

[c-Fos]

other gene names :

[FOS; FOS; p55; AP-1; C-FOS; G0S7]

uniprot entry name :

FOS_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[7D6-A9-D4]

host :

Mouse

reactivity :

Human, Rat, Monkey

sequence length :

380

specificity :

This antibody detects endogenous levels of FOS and does not cross-react with related proteins.

purity :

Affinity purified

form :

Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20 degree C

tested application :

Western Blot (WB)

app notes :

Western blotting: 1:500

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

other info1 :

Immunogen Type: Recombinant Protein. Immunogen Description: Purified recombinant human FOS protein fragments expressed in E Coli.

other info2 :

Target Name: c-Fos

products categories :

Total protein Ab

products description :

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor.In the heterodimer,FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites.On TGF-beta activation,forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling.Has a critical function in regulating the development of cells destined to form and maintain the skeleton.It is thought to have an important role in signal transduction,cell proliferation and differentiation.In growing cells,activates phospholipid synthesis,possibly by activating CDS1 and PI4K2A.This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor.In the heterodimer,FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites.On TGF-beta activation,forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling.Has a critical function in regulating the development of cells destined to form and maintain the skeleton.It is thought to have an important role in signal transduction,cell proliferation and differentiation.In growing cells,activates phospholipid synthesis,possibly by activating CDS1 and PI4K2A.This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

ncbi gi num :

4885241

ncbi acc num :

NP_005243.1

ncbi gb acc num :

NM_005252.3

uniprot acc num :

P01100

ncbi mol weight :

62kd

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Activated TLR4 Signalling Pathway (1269236); Activation Of The AP-1 Family Of Transcription Factors Pathway (1269218); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Apoptosis Modulation And Signaling Pathway (198822); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); BDNF Signaling Pathway (712093)

ncbi summary :

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]

uniprot summary :

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

255

more info or order :

MyBioSource product webpage