Histone H3 Mouse Monoclonal Antibody | MyBioSource MBS9400490 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Histone H3 Mouse Monoclonal Antibody

catalog :

MBS9400490

quantity :

0.05 mL

price :

200 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue, using MBS9400490 diluted at 1:5,000.

image 2 :

IF analysis of Hela with DAPI (Left) and MBS9400490(Right) diluted at 1:200.

image 3 :

Input: Hela Cell Lysate. 2B'B'IP product: IP dilute 1:200. Western blot analysis: primary antibody MBS9400490: 1:2,000. Secondary antibody: Goat anti-Mouse IgG(H+L) 1:10,000.

product information

catalog number :

MBS9400490

products type :

Antibody

products full name :

Histone H3 Mouse Monoclonal Antibody

products short name :

[Histone H3]

products name syn :

[H3/A; H3FA; H3FB; H3FC]

other names :

[histone H3.1; Histone H3.1; histone H3.1; histone cluster 1, H3a; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l]

other gene names :

[HIST1H3A; HIST1H3A; H3/A; H3FA; H3FA]

uniprot entry name :

H31_HUMAN

clonality :

Monoclonal

host :

Mouse

reactivity :

Human, Rat, Mouse

sequence length :

136

specificity :

The Histone H3 antibody can detect endogenous Histone H3 protein.

purity :

Affinity purification using immunogen.

form :

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0 mg/ml

storage stability :

Store at -20 degree C

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

app notes :

Western blotting: 1:2000~1:5000. Immunofluorescence: 1:100-500. Immunoprecipitation: 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Testing Data

other info2 :

Target Name: Histone H3

products categories :

Total protein Ab

products description :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

ncbi gi num :

4504281

ncbi acc num :

NP_003520.1

ncbi gb acc num :

NM_003529.2

uniprot acc num :

P68431

ncbi mol weight :

17 kd

ncbi pathways :

Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway (1269513); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amyloids Pathway (1269169); Cell Cycle Pathway (1269741); Cell Cycle, Mitotic Pathway (1269763); Cellular Senescence Pathway (1270426); Cellular Responses To Stress Pathway (1270414); Chromatin Modifying Enzymes Pathway (1270434); Chromatin Organization Pathway (1270433)

ncbi summary :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

uniprot summary :

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 6p22.2. Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus. Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity. Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; regulation of gene expression, epigenetic; DNA methylation on cytosine; DNA replication-dependent nucleosome assembly

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

255

more info or order :

MyBioSource product webpage