product summary
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company name :
MyBioSource
product type :
antibody
product name :
Flag-Tag Mouse Monoclonal Antibody
catalog :
MBS9400453
quantity :
0.05 mL
price :
200 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
application :
western blot, immunocytochemistry, immunoprecipitation
more info or order :
image
image 1 :
1ug Flag fusion protein+ Primary antibody dilution at 1:10,000.
image 2 :
IF analysis of 293 cells transfected with a Flag-tag protein, using anti-Flag-Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag).
image 3 :
IP antibody use 15 ug Flag Mouse IgG1 per ml. Lysate WB 1:5000. 1 B'B untransfected 293 cell lysate. 2 B'B transfected 293 cell lysate with Flag-tag fusion protein . 3 B'B'IP(transfected 293+ normal Mouse IgG+Protein G agarose) . 4 B'B'IP (transfected 293+anti- Flag mAb+ Protein G agarose). 5 B'B'IP(transfected 293+Protein G) . 6 B'B'Recombinant protein (E Coli).
product information
catalog number :
MBS9400453
products type :
Antibody
products full name :
Flag-Tag Mouse Monoclonal Antibody
products short name :
[Flag-Tag]
clonality :
Monoclonal
host :
Mouse
reactivity :
Human, Mouse, Rat
purity :
Affinity purification using immunogen.
form :
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
concentration :
1.0 mg/ml
storage stability :
Store at -20°C
tested application :
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
app notes :
Western blotting: 1:5000~1:10000. Immunofluorescence: 1:2000. Immunoprecipitation: 1:5000
image1 heading :
Testing Data
image2 heading :
Immunofluorescence (IF)
image3 heading :
Immunoprecipitation (IP)
other info2 :
Target Name: Flag-Tag. Specifity: The Flag tag antibody can recognize C-terminal, internal and N-terminal Flag-tag fusion proteins.
products categories :
Tag Ab
products description :
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
size1 :
0.05 mL
price1 :
200 USD
size2 :
0.1 mL
price2 :
255
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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