antibody

KLF4 Monoclonal Antibody

MBS9400367

0.1 mL

320 USD

monoclonal

mouse

nonconjugated

[4G6E11]

human, mouse, rat

western blot, ELISA, enzyme immunoassay

Antibody

KLF4 Monoclonal Antibody

[KLF4]

[Kruppel-like factor 4, GKLF, EZF]

[KLF4 protein; Krueppel-like factor 4; Krueppel-like factor 4; Kruppel-like factor 4 (gut); Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor]

[KLF4]

[KLF4; KLF4; EZF; GKLF; EZF; GKLF]

KLF4_HUMAN

Monoclonal

[4G6E11]

Mouse

Human, Mouse, Rat

504

At least three isoforms of KLF4 are known to exist; this antibody will detect all three. KLF4 antibody will not cross-react with other Kruppel-like family members.

Affinity chromatography purified via peptide column

Supplied in PBS containing 0.02% sodium azide.

Can be stored at -20 degree C, stable for one year.

ELISA (EIA), Western Blot (WB)

Western Blot (WB)

Immunogen Type: Peptide. Immunogen Description: Raised against a 20 amino acid peptide near the carboxy terminus of human KLF4.

Target Name: KLF4

Total protein Ab

KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.

21410813

AAH30811

6,489 Da

Developmental Biology Pathway (1270302); Metabolism Of Proteins Pathway (1268677); Peptide Hormone Metabolism Pathway (1268746); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway (672464); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026136); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502); Synthesis, Secretion, And Deacylation Of Ghrelin Pathway (1268748); Transcriptional Regulation Of Pluripotent Stem Cells Pathway (1270346); Transcriptional Regulation Of White Adipocyte Differentiation Pathway (1270345)

KLF4: Transcription factor; can act both as activator and as repressor. Binds the 5 -CACCC-3 core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 9q31. Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin. Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; phosphoinositide 3-kinase regulator activity; transcription factor activity. Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; somatic stem cell maintenance; negative regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein metabolic process; regulation of phosphoinositide 3-kinase activity; positive regulation of hemoglobin biosynthetic process; negative regulation of cell proliferation; post-embryonic hemopoiesis; regulation of cell differentiation; epidermis morphogenesis; response to retinoic acid; negative regulation of protein kinase B signaling cascade; negative regulation of heterotypic cell-cell adhesion; stem cell maintenance; positive regulation of telomerase activity; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; mesodermal cell fate determination; negative regulation of phosphoinositide 3-kinase cascade

0.1 mL

320 USD