catalog number :
MBS939860
products type :
ELISA Kit
products full name :
Sheep Calponin-1, CNN1 ELISA Kit
products short name :
calponin 1, basic, smooth muscle
products name syn :
Sheep Calponin-1 (CNN1) ELISA kit; SMCC; Sm-Calp; calponins; basic; calponin 1; basic; smooth muscle
other names :
calponin-1; Calponin-1; calponin-1; basic calponin; smooth muscle calponin h1; calponin H1, smooth muscle; Basic calponin; Calponin H1, smooth muscle
products gene name :
CNN1
other gene names :
CNN1; CNN1
uniprot entry name :
CNN1_SHEEP
specificity :
This assay has high sensitivity and excellent specificity for detection of sheep CNN1. No significant cross-reactivity or interference between sheep CNN1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of sheep CNN1 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CNN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CNN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CNN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CNN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001009456.1
ncbi gb acc num :
NM_001009456.1
ncbi mol weight :
33,255 Da
uniprot summary :
Function: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity
size4 :
10x96-Strip-Wells