Mouse Sortilin, SORT1 ELISA Kit | MyBioSource MBS939726 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Sortilin, SORT1 ELISA Kit

catalog :

MBS939726

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS939726

products type :

ELISA Kit

products full name :

Mouse Sortilin, SORT1 ELISA Kit

products short name :

sortilin 1

products name syn :

Mouse Sortilin (SORT1) ELISA kit; Gp95; NT3; neurotensin receptor 3; sortilin 1

other names :

sortilin isoform 1; Sortilin; sortilin; mNTR3; neurotensin receptor 3; sortilin 1; Neurotensin receptor 3; NTR3; mNTR3

products gene name :

SORT1

other gene names :

Sort1; Sort1; Ntr3; Ntsr3; AI852375; 2900053A11Rik; NTR3; mNTR3

uniprot entry name :

SORT_MOUSE

reactivity :

Mouse

sequence length :

825

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse SORT1. No significant cross-reactivity or interference between mouse SORT1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml-400 pg/ml. Sensitivity: 1.56 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SORT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SORT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

409264681

ncbi acc num :

NP_001258528.1

ncbi gb acc num :

NM_001271599.1

uniprot acc num :

Q6PHU5

ncbi mol weight :

91,200 Da

ncbi pathways :

Clathrin Derived Vesicle Budding Pathway (574572); Golgi Associated Vesicle Biogenesis Pathway (574573); Lysosome Pathway (99272); Lysosome Pathway (96865); Membrane Trafficking Pathway (574566); Neurotrophin Signaling Pathway (101156); Neurotrophin Signaling Pathway (100064); Trans-Golgi Network Vesicle Budding Pathway (574571)

uniprot summary :

SORT1: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. A common polymorphism located in a non-coding region between CELSR2 and PSRC1 alters a CEBP transcription factor binding site and is responsible for changes in hepatic expression of SORT1. Altered SORT1 expression in liver affects low density lipoprotein cholesterol levels in plasma and is associated with susceptibility to myocardial infarction. Belongs to the VPS10-related sortilin family. SORT1 subfamily. Protein type: Membrane protein, integral; Receptor, misc. Cellular Component: Golgi apparatus; cell surface; intracellular membrane-bound organelle; clathrin-coated vesicle; endoplasmic reticulum; lysosome; early endosome; dendrite; cytoplasmic membrane-bound vesicle; integral to membrane; coated pit; trans-Golgi network transport vesicle; membrane; cell soma; perinuclear region of cytoplasm; plasma membrane; nucleus; endosome. Molecular Function: protein binding; enzyme binding; nerve growth factor receptor activity; nerve growth factor binding; neurotensin receptor activity, non-G-protein coupled. Biological Process: ossification; nerve growth factor receptor signaling pathway; endosome transport via multivesicular body sorting pathway; multicellular organismal development; vesicle organization and biogenesis; Golgi to endosome transport; myotube differentiation; endocytosis; response to insulin stimulus; G-protein coupled receptor protein signaling pathway; induction of apoptosis via death domain receptors; neuropeptide signaling pathway; regulation of gene expression; transport; glucose import; endosome to lysosome transport; plasma membrane to endosome transport; negative regulation of lipoprotein lipase activity; cell differentiation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage