catalog number :
MBS939515
products type :
ELISA Kit
products full name :
Mouse Kallikrein-1, KLK1 ELISA Kit
products short name :
[kallikrein 1]
products name syn :
[Mouse Kallikrein-1 (KLK1) ELISA kit; KLKR; Klk6; hK1; glandular kallikrein 1; kallikrein 1; renal/pancreas/salivary; kallikrein serine protease 1; tissue kallikrein; kallikrein 1]
other names :
[kallikrein-1; Kallikrein-1; kallikrein-1; KAL-B; kallikrein 6; renal kallikrein; tissue kallikrein-6; true tissue kallikrein; glandular kallikrein K1; kallikrein renal/pancreas/salivary; kallikrein 1; Glandular kallikrein K1; KAL-B; Renal kallikrein; Tissue kallikrein-6; mGK-6]
products gene name :
[KLK1]
other gene names :
[Klk1; Klk1; Kal; Klk6; mGk-6; Klk1b6; 0610007D04Rik; Klk-6; Klk6; mGK-6]
uniprot entry name :
KLK1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of human KLF2. No significant cross-reactivity or interference between human KLF2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KLF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_034769.4
ncbi gb acc num :
NM_010639.7
ncbi mol weight :
28,775 Da
ncbi pathways :
Diabetes Pathways (574950); Disease Pathway (574923); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213309); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276); Regulation Of Insulin-like Growth Factor (IGF) Activity By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (574956)
uniprot summary :
KLK1: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.21.35; Protease. Cellular Component: nucleus. Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity. Biological Process: proteolysis
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells