catalog number :
MBS939371
products type :
ELISA Kit
products full name :
Mouse Roundabout homolog 4, ROBO4 ELISA Kit
products short name :
roundabout homolog 4, magic roundabout (Drosophila)
products name syn :
Mouse Roundabout homolog 4 (ROBO4) ELISA kit; ECSM4; FLJ20798; MGC133352; MGC133353; MRB; roundabout homolog 4; magic roundabout; roundabout homolog 4; magic roundabout (Drosophila)
other names :
roundabout homolog 4; Roundabout homolog 4; roundabout homolog 4; Magic roundabout; roundabout homolog 4 (Drosophila)
products gene name :
ROBO4
other gene names :
Robo4; Robo4; AI593217; 1200012D01Rik
uniprot entry name :
ROBO4_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse ROBO4. No significant cross-reactivity or interference between Mouse ROBO4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ROBO4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ROBO4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ROBO4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ROBO4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_083059.2
ncbi gb acc num :
NM_028783.2
ncbi mol weight :
108,499 Da
uniprot summary :
ROBO4: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Belongs to the immunoglobulin superfamily. ROBO family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Cellular Component: integral to membrane; external side of plasma membrane. Molecular Function: protein binding; receptor activity. Biological Process: multicellular organismal development; angiogenesis; cell differentiation; negative regulation of cell migration
size4 :
10x96-Strip-Wells