Human Interferon regulatory factor 7, IRF7 ELISA Kit | MyBioSource MBS939129 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Interferon regulatory factor 7, IRF7 ELISA Kit

catalog :

MBS939129

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS939129

products type :

ELISA Kit

products full name :

Human Interferon regulatory factor 7, IRF7 ELISA Kit

products short name :

interferon regulatory factor 7

products name syn :

Human Interferon regulatory factor 7 (IRF7) ELISA kit; IRF-7H; IRF7A; OTTHUMP00000162886; OTTHUMP00000162888; interferon regulatory factor-7H; interferon regulatory factor 7

other names :

interferon regulatory factor 7 isoform a; Interferon regulatory factor 7; interferon regulatory factor 7; IRF-7; interferon regulatory factor-7H; interferon regulatory factor 7

products gene name :

IRF7

other gene names :

IRF7; IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H; IRF-7

uniprot entry name :

IRF7_HUMAN

reactivity :

Human

sequence length :

503

specificity :

This assay has high sensitivity and excellent specificity for detection of Human IRF7. No significant cross-reactivity or interference between Human IRF7 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.4 pg/ml-1500 pg/ml. Sensitivity: 5.8 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Antiviral defense

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IRF7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IRF7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IRF7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IRF7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4758610

ncbi acc num :

NP_001563.2

ncbi gb acc num :

NM_001572.3

uniprot acc num :

Q92985

ncbi mol weight :

54,278 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (106400); Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway (187088); Apoptosis Pathway (198797); Cytokine Signaling In Immune System Pathway (366171); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907)

ncbi summary :

IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq, Jul 2008]

uniprot summary :

IRF7: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88- independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages. Monomer. Homodimer; phosphorylation-induced. Heterodimer with IRF3. Interacts with TICAM1 and TICAM2. Interacts with rotavirus A NSP1; this interaction leads to the proteasome- dependent degradation of IRF7. Interacts with Epstein-Barr virus LF2. Interacts with MYD88 AND TRAF6. By type I interferon (IFN) and viruses. Expressed predominantly in spleen, thymus and peripheral blood leukocytes. In the absence of viral infection, maintained as a monomer in an autoinhibited state and phosphorylation disrupts this autoinhibition leading to the liberation of the DNA- binding and dimerization activities and its nuclear localization where it can activate type I IFN and ISG genes. Belongs to the IRF family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: nucleoplasm; cytoplasm; endosome membrane; nucleus; cytosol. Molecular Function: protein binding; DNA binding. Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; establishment of viral latency; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; regulation of adaptive immune response; regulation of MyD88-independent toll-like receptor signaling pathway; positive regulation of interferon-beta production; positive regulation of interferon type I production; interferon type I biosynthetic process; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; regulation of interferon type I production; regulation of immune response; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; response to virus; interferon-alpha production; regulation of MyD88-dependent toll-like receptor signaling pathway; regulation of monocyte differentiation; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; immunoglobulin mediated immune response; response to DNA damage stimulus; interferon-beta production. Disease: Immunodeficiency 39

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage