Mouse Elastin, ELN ELISA Kit | MyBioSource MBS939028 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Elastin, ELN ELISA Kit

catalog :

MBS939028

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS939028

products type :

ELISA Kit

products full name :

Mouse Elastin, ELN ELISA Kit

products short name :

[elastin]

products name syn :

[Mouse Elastin (ELN) ELISA kit; FLJ38671; FLJ43523; SVAS; WBS; WS; OTTHUMP00000211864; tropoelastin; elastin]

other names :

[elastin; Elastin; elastin; tropoelastin; elastin; Tropoelastin]

products gene name :

[ELN]

other gene names :

[Eln; Eln; AI385707; AI480567; E030024M20Rik]

uniprot entry name :

ELN_MOUSE

reactivity :

Mouse

sequence length :

860

specificity :

This assay has high sensitivity and excellent specificity for detection of human ELN. No significant cross-reactivity or interference between human ELN and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, Plasma, Cell Culture Supernates, Urine, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: 0.78 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ELN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ELN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELN bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

31542606

ncbi acc num :

NP_031951.2

ncbi gb acc num :

NM_007925.3

uniprot acc num :

P54320

ncbi mol weight :

71,938 Da

ncbi pathways :

Protein Digestion And Absorption Pathway (172853); Protein Digestion And Absorption Pathway (171868)

ncbi summary :

This gene encodes elastin, the extracellular matrix protein that forms a major structural component of several tissues including lungs and arterial walls. Cleavage of the signal peptide from the encoded precursor generates soluble tropoelastin which undergoes lysine-derived crosslinking to form elastin polymers. Mice lacking the encoded protein exhibit defective lung development, and die of an obstructive arterial disease resulting from subendothelial cell proliferation and reorganization of smooth muscle. [provided by RefSeq, Aug 2015]

uniprot summary :

elastin: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. Defects in ELN are the cause of cutis laxa, autosomal dominant, type 1 (ADCL1). A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema. Defects in ELN are the cause of supravalvular aortic stenosis (SVAS). SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome. ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. Belongs to the elastin family. 13 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Extracellular matrix. Cellular Component: proteinaceous extracellular matrix; mitochondrion; extracellular region. Molecular Function: protein binding; extracellular matrix binding; extracellular matrix structural constituent. Biological Process: regulation of actin filament polymerization; extracellular matrix organization and biogenesis; skeletal muscle development; stress fiber formation

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145

more info or order :

MyBioSource product webpage