Human thiopurine S-methyltransferase, TPMT ELISA Kit | MyBioSource MBS938845 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human thiopurine S-methyltransferase, TPMT ELISA Kit

catalog :

MBS938845

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS938845

products type :

ELISA Kit

products full name :

Human thiopurine S-methyltransferase, TPMT ELISA Kit

products short name :

thiopurine S-methyltransferase

products name syn :

Human thiopurine S-methyltransferase (TPMT) ELISA kit; S-adenosyl-L-methionine:thiopurine S-methyltransferase; thiopurine S-methyltransferase

other names :

thiopurine S-methyltransferase; Thiopurine S-methyltransferase; thiopurine S-methyltransferase; S-adenosyl-L-methionine:thiopurine S-methyltransferase; thiopurine S-methyltransferase; Thiopurine methyltransferase

products gene name :

TPMT

other gene names :

TPMT; TPMT

uniprot entry name :

TPMT_HUMAN

reactivity :

Human

sequence length :

245

specificity :

This assay has high sensitivity and excellent specificity for detection of human TPMT. No significant cross-reactivity or interference between human TPMT and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 mU/ml -100 mU/ml. Sensitivity: The minimum detectable dose of human TPMT is typically less than 0.39 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPMT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPMT bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4507653

ncbi acc num :

NP_000358.1

ncbi gb acc num :

NM_000367.3

uniprot acc num :

P51580

ncbi mol weight :

28,180 Da

ncbi pathways :

Biological Oxidations Pathway (105698); Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Metabolism Pathway (477135); Methylation Pathway (105726); Phase II Conjugation Pathway (105721)

ncbi summary :

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]

uniprot summary :

TPMT: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Defects in TPMT are the cause of thiopurine S- methyltransferase deficiency (TPMT deficiency). TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Belongs to the methyltransferase superfamily. TPMT family. Protein type: Methyltransferase; EC 2.1.1.67; Xenobiotic Metabolism - drug metabolism - other enzymes. Chromosomal Location of Human Ortholog: 6p22.3. Cellular Component: cytosol. Molecular Function: thiopurine S-methyltransferase activity. Biological Process: methylation; xenobiotic metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process. Disease: Thiopurine S-methyltransferase Deficiency

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage