Phospho-Beclin-1 (Ser93/96) Antibody | MyBioSource MBS9386755 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-Beclin-1 (Ser93/96) Antibody

catalog :

MBS9386755

quantity :

0.1 mL

price :

440 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Phospho-Beclin-1 (Ser93/96) usingK562 whole cell lysates

product information

catalog number :

MBS9386755

products type :

Antibody

products full name :

Phospho-Beclin-1 (Ser93/96) Antibody

products short name :

[Beclin-1]

other names :

[beclin-1 isoform a; Beclin-1; beclin-1; beclin 1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197]

other gene names :

[BECN1; BECN1; ATG6; VPS30; beclin1; GT197]

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

450

specificity :

Phospho-Beclin-1 (Ser93/96) Ab detects endogenous levels of Beclin-1 only when phosphorylated at Ser93/96

purity :

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and nonphospho- peptide affinity columns.

concentration :

1 mg/ml

storage stability :

Store at -20°C.Stable for 12 months from date of receipt

tested application :

Western Blot (WB)

app notes :

WB 1:1000-3000

image1 heading :

Western Blot (WB)

other info1 :

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Immunogen: A synthesized peptide derived from human Phospho-Beclin-1(Ser93/96). Subcellular location: Golgi apparatus > trans-Golgi network membrane.Interaction with ATG14 promotes translocation toautophagosomes. Expressed in dendrites and cell bodies ofcerebellar Purkinje cells. Tissue specifity: Ubiquitous. Similarity: The coiled coil domain can form antiparallel homodimers and mediates dimerization with the coiled coil domains of ATG14 or UVRAG involved in the formation of PI3K complexes.The C-terminal evolutionary conserved domain (ECD) contains poly-Gln-binding domains such as the ATXN3 poly-Gln motif, consistent with structural docking models revealing two highly scored poly-Gln-binding pockets in the ECD (PubMed:28445460). As some binding is observed with BECN1 lacking the ECD, other domains of BECN1 may also interact with ATXN3 (PubMed:28445460).Belongs to the beclin family.

ncbi gi num :

929524267

ncbi acc num :

NP_001300927.1

ncbi gb acc num :

NM_001313998.1

uniprot acc num :

Q14457

ncbi mol weight :

60 KD

ncbi pathways :

Apoptosis - Multiple Species Pathway (1384747); Apoptosis - Multiple Species Pathway (1384709); Autophagy Pathway (83058); Autophagy Pathway (468); Cellular Responses To Stress Pathway (1270414); Deubiquitination Pathway (1427860); Macroautophagy Pathway (1270432); Metabolism Of Proteins Pathway (1268677); Nanoparticle Triggered Autophagic Cell Death Pathway (1458208); Post-translational Protein Modification Pathway (1268701)

ncbi summary :

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

uniprot summary :

beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. Protein type: Adaptor/scaffold; Autophagy; Membrane protein, peripheral. Chromosomal Location of Human Ortholog: 17q21.31. Cellular Component: cytosol; endoplasmic reticulum; endosome; extrinsic to membrane; mitochondrion. Molecular Function: phosphoinositide 3-kinase binding; protein binding; ubiquitin protein ligase binding. Biological Process: autophagic vacuole formation; autophagy; cellular defense response; cellular response to glucose starvation; cellular response to nitrogen starvation; CVT pathway; cytokinesis; late endosome to vacuole transport; macroautophagy; mitotic metaphase plate congression; positive regulation of autophagy; receptor catabolic process; regulation of cytokinesis

size1 :

0.1 mL

price1 :

440 USD

size2 :

0.2 mL

price2 :

715

more info or order :

MyBioSource product webpage