Mouse Ubiquitin-like protein ISG15, ISG15 ELISA Kit | MyBioSource MBS938670 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Ubiquitin-like protein ISG15, ISG15 ELISA Kit

catalog :

MBS938670

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS938670

products type :

ELISA Kit

products full name :

Mouse Ubiquitin-like protein ISG15, ISG15 ELISA Kit

products short name :

ISG15 ubiquitin-like modifier

products name syn :

Mouse Ubiquitin-like protein ISG15 (ISG15) ELISA kit; G1P2; IFI15; UCRP; interferon; alpha-inducible protein (clone IFI-15K) ; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein; 15 kDa; ubiquitin cross-reactive protein; ISG15 ubiquitin-like modifier

other names :

ubiquitin-like protein ISG15; Ubiquitin-like protein ISG15; ubiquitin-like protein ISG15; interferon-stimulated protein 15; ubiquitin cross-reactive protein; interferon-induced 15 kDa protein; interferon-induced 15-KDa protein; interferon-induced 17 kDa protein; interferon, alpha-inducible protein; interferon-stimulated protein (15 kDa); ISG15 ubiquitin-like modifier; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein

products gene name :

ISG15

other gene names :

Isg15; Isg15; G1p2; IP17; Irfp; UCRP; IGI15; G1p2; Ucrp; IP17

uniprot entry name :

ISG15_MOUSE

reactivity :

Mouse

sequence length :

161

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse ISG15. No significant cross-reactivity or interference between mouse ISG15 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of mouse ISG15 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ISG15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ISG15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ISG15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ISG15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

226874851

ncbi acc num :

NP_056598.2

ncbi gb acc num :

NM_015783.3

uniprot acc num :

Q64339

ncbi mol weight :

17,898 Da

ncbi pathways :

Immune System Pathway (575095); Innate Immune System Pathway (575115); RIG-I-like Receptor Signaling Pathway (117302); RIG-I-like Receptor Signaling Pathway (116128); RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways (575158); Type II Interferon Signaling (IFNG) Pathway (198408)

uniprot summary :

ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. Protein type: Ubiquitin-like modifier. Cellular Component: cytoplasm; extracellular region. Molecular Function: protein tag; protein binding. Biological Process: ISG15-protein conjugation; modification-dependent protein catabolic process; negative regulation of viral genome replication; regulation of interferon-gamma production; positive regulation of erythrocyte differentiation; defense response to bacterium; negative regulation of protein ubiquitination; defense response to virus

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage