antibody

MLK3 (Phospho-Thr277+Ser281) Antibody

MBS9386068

0.1 mL

440 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

Antibody

MLK3 (Phospho-Thr277+Ser281) Antibody

[MLK3]

[mitogen-activated protein kinase kinase kinase 11; Mitogen-activated protein kinase kinase kinase 11; mitogen-activated protein kinase kinase kinase 11; mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase]

[MLK3]

[MAP3K11; MAP3K11; MLK3; PTK1; SPRK; MLK-3; MEKK11]

Polyclonal

Rabbit

Human, Mouse, Rat

847

MLK3 (Phospho-Thr277+Ser281) Ab detects endogenous levels of MLK3 only when phosphorylated at Thr277+Ser281

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and nonphospho-peptide affinity columns.

1.0mg/ml

Store at -20°C.Stable for 12 months from date of receipt

Western Blot (WB)

WB 1:1000-3000. IHC 1:50-1:200

Western Blot (WB)

Subcellular location: Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent. Immunogen: A synthesized peptide derived from human MLK3 (Phospho-Thr277+Ser281). Tissue Specifity: Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

4505195

NP_002410.1

NM_002419.3

Q16584

92 KD

CDC42 Signaling Events Pathway (137994); IFN-gamma Pathway (138040); Insulin Signaling Pathway (198845); MAPK Signaling Pathway (198779); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862188); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862314); RAC1 Signaling Pathway (169355); Reelin Signaling Pathway (137980)

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]

MLK3: a TKL kinase of the MLK family. Has an amino-terminal SH3 domain followed by the kinase domain, two leucine zipper domains, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. Phosphorylates IkappaB and activates SEK1 and MKK7. Protein type: EC 2.7.11.25; Kinase, protein; MLK family; MLK subfamily; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group. Chromosomal Location of Human Ortholog: 11q13.1. Cellular Component: centrosome; cytoplasm; membrane; microtubule. Molecular Function: identical protein binding; JUN kinase kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity. Biological Process: activation of JNK activity; activation of MAPK activity; cell proliferation; JNK cascade; microtubule-based process; positive regulation of apoptosis; positive regulation of JNK activity; positive regulation of JNK cascade; protein amino acid autophosphorylation; protein amino acid phosphorylation

0.1 mL

440 USD

0.2 mL

715