ELISA/assay

Human Cystatin-SN, CST1 ELISA Kit

MBS938558

48-Strip-Wells

510 USD

ELISA Kit

Human Cystatin-SN, CST1 ELISA Kit

cystatin SN

Human Cystatin-SN (CST1) ELISA kit; OTTHUMP00000164184; cystatin 1; cystatin SA-I; cysteine proteinase inhibitor; type 2 family; cystatin SN

cystatin-SN; Cystatin-SN; cystatin-SN; cystatin 1; cystatin-1; cystain-SA-I; cystatin SA-I; salivary cystatin-SA-1; cysteine proteinase inhibitor, type 2 family; cystatin SN; Cystain-SA-I; Cystatin-1; Salivary cystatin-SA-1

CST1

CST1; CST1

CYTN_HUMAN

Human

141

This assay has high sensitivity and excellent specificity for detection of human CST1. No significant cross-reactivity or interference between human CST1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, urine, saliva, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CST1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CST1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CST1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CST1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

19882251

NP_001889.2

NM_001898.2

P01037

16,388 Da

Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid. [provided by RefSeq, Jul 2008]

CST1: Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well. Belongs to the cystatin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20p11.21. Cellular Component: extracellular space. Molecular Function: cysteine protease inhibitor activity. Biological Process: detection of chemical stimulus involved in sensory perception of bitter taste

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800