Human Gamma-aminobutyric acid receptor-associated protein, GABARAP ELISA Kit | MyBioSource MBS938530 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Gamma-aminobutyric acid receptor-associated protein, GABARAP ELISA Kit

catalog :

MBS938530

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS938530

products type :

ELISA Kit

products full name :

Human Gamma-aminobutyric acid receptor-associated protein, GABARAP ELISA Kit

products short name :

[GABA (A) receptor-associated protein]

products name syn :

[Human Gamma-aminobutyric acid receptor-associated protein (GABARAP) ELISA kit; FLJ25768; MGC120154; MGC120155; MM46; ; GABA (A) receptor-associated protein]

other names :

[gamma-aminobutyric acid receptor-associated protein; Gamma-aminobutyric acid receptor-associated protein; gamma-aminobutyric acid receptor-associated protein; GABA(A) receptor-associated protein; GABA(A) receptor-associated protein; MM46]

products gene name :

[GABARAP]

other gene names :

[GABARAP; GABARAP; MM46; ATG8A; GABARAP-a; FLC3B; HT004]

uniprot entry name :

GBRAP_HUMAN

reactivity :

Human

sequence length :

117

specificity :

This assay has high sensitivity and excellent specificity for detection of human GABARAP. No significant cross-reactivity or interference between human GABARAP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 9.77 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GABARAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GABARAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GABARAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GABARAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6005764

ncbi acc num :

NP_009209.1

ncbi gb acc num :

NM_007278.1

uniprot acc num :

O95166

ncbi mol weight :

13,918 Da

ncbi pathways :

GABAergic Synapse Pathway (377263); GABAergic Synapse Pathway (377129); Regulation Of Autophagy Pathway (83058); Regulation Of Autophagy Pathway (468); Senescence And Autophagy Pathway (198780)

ncbi summary :

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008]

uniprot summary :

GABARAP: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Belongs to the MAP1 LC3 family. Interacts with GPHN and NSF. Interacts with GABRG2, beta-tubulin and ULK1. Protein type: Autophagy; Vesicle; Microtubule-binding; Ubiquitin-like modifier; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: Golgi membrane; microtubule; microtubule associated complex; smooth endoplasmic reticulum; extrinsic to membrane; perinuclear region of cytoplasm; lysosome; plasma membrane; autophagic vacuole; cytoplasmic vesicle; axoneme; cytosol; actin cytoskeleton. Molecular Function: protein binding; microtubule binding; beta-tubulin binding; GABA receptor binding. Biological Process: synaptic transmission; induction of apoptosis via death domain receptors; mitochondrion degradation; microtubule cytoskeleton organization and biogenesis; protein targeting; autophagic vacuole formation

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145

more info or order :

MyBioSource product webpage