ELISA/assay

Human Beta-1, 4-galactosyltransferase 1, B4GALT1 ELISA Kit

MBS938479

48-Strip-Wells

510 USD

ELISA Kit

Human Beta-1, 4-galactosyltransferase 1, B4GALT1 ELISA Kit

UDP-Gal:betaGlcNAc beta 1, 4- galactosyltransferase, polypeptide 1

Human Beta-1; 4-galactosyltransferase 1 (B4GALT1) ELISA kit; B4GAL-T1; DKFZp686N19253; GGTB2; GT1; GTB; MGC50983; beta4Gal-T1; beta-1; 4-galactosyltransferase 1; glycoprotein-4-beta-galactosyltransferase 2; lactose synthase; UDP-Gal:betaGlcNAc beta 1; 4- galactosyltransferase; polypeptide 1

beta-1,4-galactosyltransferase 1; Beta-1,4-galactosyltransferase 1; beta-1,4-galactosyltransferase 1; lactose synthase; beta-1,4-GalTase 1; glycoprotein-4-beta-galactosyltransferase 2; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1Cleaved into the following chain:Processed beta-1,4-galactosyltransferase 1Including the following 4 domains:Lactose synthase A protein (EC:2.4.1.22); N-acetyllactosamine synthase (EC:2.4.1.90)Alternative name(s):Nal synthase

B4GALT1

B4GALT1; B4GALT1; GT1; GTB; CDG2D; GGTB2; B4GAL-T1; beta4Gal-T1; GGTB2; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1

B4GT1_HUMAN

Human

398

This assay has high sensitivity and excellent specificity for detection of human B4GALT1. No significant cross-reactivity or interference between human B4GALT1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human B4GALT1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for B4GALT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any B4GALT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for B4GALT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of B4GALT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

13929462

NP_001488.2

NM_001497.3

P15291

43,920 Da

Asparagine N-linked Glycosylation Pathway (161013); Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292); Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway (82983); Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway (357); Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (82995); Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (370); Glycosphingolipid Biosynthesis, Neolacto-series, LacCer = NLc4Cer Pathway (413372); Glycosphingolipid Biosynthesis, Neolacto-series, LacCer = NLc4Cer Pathway (468264); Metabolism Of Proteins Pathway (106230)

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase. [provided by RefSeq, Jul 2008]

B4GALT1: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Defects in B4GALT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the glycosyltransferase 7 family. 2 isoforms of the human protein are produced by alternative initiation. Protein type: EC 2.4.1.38; EC 2.4.1.22; Transferase; Cell adhesion; Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral; Carbohydrate Metabolism - galactose; Cell surface; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Cell development/differentiation; Motility/polarity/chemotaxis; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.90. Chromosomal Location of Human Ortholog: 9p13. Cellular Component: glycocalyx; Golgi apparatus; desmosome; extracellular space; Golgi trans cisterna; basolateral plasma membrane; brush border membrane; integral to membrane; Golgi membrane; membrane; plasma membrane; filopodium; external side of plasma membrane. Molecular Function: beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; protein homodimerization activity; N-acetyllactosamine synthase activity; manganese ion binding; lactose synthase activity; cytoskeletal protein binding; beta-tubulin binding; UDP-galactosyltransferase activity; galactosyltransferase activity; alpha-tubulin binding. Biological Process: keratan sulfate metabolic process; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; positive regulation of epithelial cell proliferation involved in wound healing; oligosaccharide biosynthetic process; positive regulation of apoptosis involved in mammary gland involution; regulation of acrosome reaction; pathogenesis; lactose biosynthetic process; negative regulation of cell proliferation; mammary gland development; development of secondary sexual characteristics; single fertilization; epithelial cell development; penetration of zona pellucida; cell adhesion; Notch signaling pathway; binding of sperm to zona pellucida; protein amino acid N-linked glycosylation; regulation of cell motility; angiogenesis involved in wound healing; post-translational protein modification; multicellular organism reproduction; cellular protein metabolic process; branching morphogenesis of a tube; keratan sulfate biosynthetic process; carbohydrate metabolic process; galactose metabolic process; protein amino acid N-linked glycosylation via asparagine; leukocyte migration; acute inflammatory response. Disease: Congenital Disorder Of Glycosylation, Type Iid

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800