DYRK1A/B (Phospho-Tyr321/273) Antibody | MyBioSource MBS9384609 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

DYRK1A/B (Phospho-Tyr321/273) Antibody

catalog :

MBS9384609

quantity :

0.1 mL

price :

440 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of DYRK1A/B (Phospho-Tyr321/273) using EGF treated K562 whole cell lysates. -/+ means absence or presence of N peptide?non-phospho-peptide) and P peptide(phospho peptide).

image 2 :

At 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.

image 3 :

At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.

product information

catalog number :

MBS9384609

products type :

Antibody

products full name :

DYRK1A/B (Phospho-Tyr321/273) Antibody

products short name :

[DYRK1A/B]

other names :

[dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform 1; Dual specificity tyrosine-phosphorylation-regulated kinase 1A; dual specificity tyrosine-phosphorylation-regulated kinase 1A; dual specificity tyrosine phosphorylation regulated kinase 1A; Dual specificity YAK1-related kinase; HP86; Protein kinase minibrain homolog; MNBH; hMNB]

products gene name :

[DYRK1A/B]

other gene names :

[DYRK1A; DYRK1A; MNB; DYRK; HP86; MNBH; MRD7; DYRK1; DYRK; MNB; MNBH; MNBH; hMNB]

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human, Mouse, Rat

specificity :

DYRK1A/B (Phospho-Tyr321/273) Ab detects endogenous levels of DYRK1A/B only when phosphorylated at Tyr321/273.

purity :

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

form :

Phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1 mg/ml

storage stability :

Store at -20°C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB 1:1000-3000, IHC 1:50-1:200. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

At 1/200 staining Human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.

other info1 :

Immunogen: A synthesized peptide derived from human DYRK1A/B around the phosphorylation site of Tyr321/273. Subcellular Location: Nucleus speckle.

other info2 :

Tissue Specificity: Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney. Similarity: The polyhistidine repeats act as targeting signals to nuclear speckles.Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

ncbi gi num :

18765758

ncbi acc num :

NP_001387.2

ncbi gb acc num :

NM_001396.4

uniprot acc num :

Q13627

ncbi mol weight :

70KD

ncbi pathways :

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); G0 And Early G1 Pathway (477139); Hedgehog Signaling Pathway (198835); Mitotic G1-G1/S Phases Pathway (160941)

ncbi summary :

This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

DYRK1A: a dual-specificity protein kinase of the DYRK family. Contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. May play a significant role in a pathway regulating cell proliferation and may be involved in brain development. Its gene is localized in the Down syndrome (DS) critical region of chromosome 21, and is a strong candidate gene for learning defects associated with Down syndrome. Overexpression in mice gives rise to neurological abnormalities similar to those observed in DS. Five splice-variant isoforms have been described. Protein type: CMGC group; DYRK family; Dyrk1 subfamily; EC 2.7.12.2; Kinase, protein; Protein kinase, CMGC; Protein kinase, dual-specificity (non-receptor). Chromosomal Location of Human Ortholog: 21q22.13. Cellular Component: nuclear speck; nucleoplasm; nucleus. Molecular Function: non-membrane spanning protein tyrosine kinase activity; protein binding; protein kinase activity; protein self-association; protein serine/threonine kinase activity; protein-tyrosine kinase activity; tau protein binding. Biological Process: circadian rhythm; negative regulation of DNA damage response, signal transduction by p53 class mediator; nervous system development; peptidyl-threonine phosphorylation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation. Disease: Mental Retardation, Autosomal Dominant 7

size1 :

0.1 mL

price1 :

440 USD

size2 :

0.2 mL

price2 :

715

more info or order :

MyBioSource product webpage