DRP1 (Phospho-Ser616) Antibody | MyBioSource MBS9384584 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

DRP1 (Phospho-Ser616) Antibody

catalog :

MBS9384584

quantity :

0.1 mL

price :

440 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of DRP1 (Phospho-Ser616) using UV treated 293 whole cell lysates

image 2 :

At 1/100 staining human Prostate carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat antirabbit Ab was used as the secondary.

image 3 :

Staining NCI-H1299 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary Ab was diluted 1/200 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H +L), diluted 1/600 was used as secondary Ab.

product information

catalog number :

MBS9384584

products type :

Antibody

products full name :

DRP1 (Phospho-Ser616) Antibody

products short name :

[DRP1]

other names :

[dynamin-1-like protein isoform 4; Dynamin-1-like protein; dynamin-1-like protein; dynamin 1 like; Dnm1p/Vps1p-like protein; DVLP; Dynamin family member proline-rich carboxyl-terminal domain less; Dymple; Dynamin-like protein; Dynamin-like protein 4; Dynamin-like protein IV; HdynIV; Dynamin-related protein 1]

products gene name :

[DRP1]

other gene names :

[DNM1L; DNM1L; DLP1; DRP1; DVLP; EMPF; EMPF1; DYMPLE; HDYNIV; DLP1; DRP1; DVLP; Dymple; HdynIV]

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

725

specificity :

DRP1 (Phospho-Ser616) Ab detects endogenous levels of DRP1 only when phosphorylated at Ser616.

purity :

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and nonphospho-peptide affinity columns.

form :

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 °C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB)

app notes :

WB: 1:1000-3000. IHC: 1:50-1:200. IF/ICC: 1:100-1:500. ELISA(peptide): 1:20000-1:40000. Important: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

image1 heading :

Western blot

image2 heading :

Immunohistochemistry

image3 heading :

Immunofluorescence

other info1 :

Immunogen: A synthesized peptide derived from human DRP1 (Phospho- Ser616).

other info2 :

Subcellular Location: Cytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex. Tissue Specificity: Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. Similarity: The GED domain folds back to interact, in cis, with the GTPbinding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization.Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

ncbi gi num :

510937022

ncbi acc num :

NP_001265392.1

ncbi gb acc num :

NM_001278463.1

uniprot acc num :

O00429

ncbi mol weight :

79kDa

ncbi pathways :

Apoptosis Pathway (105648); Apoptotic Execution Phase Pathway (105677); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590)

ncbi summary :

This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]

uniprot summary :

Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Plays an important role in mitochondrial fission during mitosis (PubMed:26992161). Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution.

size1 :

0.1 mL

price1 :

440 USD

size2 :

0.2 mL

price2 :

715

more info or order :

MyBioSource product webpage