ELISA/assay

Human Mu-type opioid receptor, OPRM1 ELISA Kit

MBS938443

48-Strip-Wells

510 USD

ELISA Kit

Human Mu-type opioid receptor, OPRM1 ELISA Kit

opioid receptor, mu 1

Human Mu-type opioid receptor (OPRM1) ELISA kit; KIAA0403; LMOR; MOR; MOR1; OPRM; mu opiate receptor; opioid receptor; mu 1

mu-type opioid receptor isoform MOR-1; Mu-type opioid receptor; mu-type opioid receptor; mu opiate receptor; mu opioid receptor hMOR-1a; opioid receptor, mu 1; Mu opiate receptor; Mu opioid receptor; MOP; hMOP

OPRM1

OPRM1; OPRM1; MOP; MOR; LMOR; MOR1; OPRM; M-OR-1; MOR1; M-OR-1; MOR-1; MOP; hMOP

OPRM_HUMAN

Human

400

This assay has high sensitivity and excellent specificity for detection of human OPRM1. No significant cross-reactivity or interference between human OPRM1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: Typically less than 7.81 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OPRM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OPRM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OPRM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OPRM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

117940060

NP_000905.3

NM_000914.4

P35372

44,779 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); G-protein Activation Pathway (106494); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); IL4-mediated Signaling Events Pathway (137933); Morphine Addiction Pathway (552665); Morphine Addiction Pathway (554808); Neuroactive Ligand-receptor Interaction Pathway (83053)

This gene encodes one of at least three opioid receptors in humans; the mu opioid receptor (MOR). The MOR is the principal target of endogenous opioid peptides and opioid analgesic agents such as beta-endorphin and enkephalins. The MOR also has an important role in dependence to other drugs of abuse, such as nicotine, cocaine, and alcohol via its modulation of the dopamine system. The NM_001008503.2:c.118A G allele has been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence for it having a causal role is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. Though the canonical MOR belongs to the superfamily of 7-transmembrane-spanning G-protein-coupled receptors some isoforms of this gene have only 6 transmembrane domains. [provided by RefSeq, Oct 2013]

MOR-1: a Gi-protein-coupled receptor for beta-endorphin, morphine and other opiates. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Ligand-binding inactivates adenylyl cyclase, and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades. Two splice-variant isoforms have been described. Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1. Chromosomal Location of Human Ortholog: 6q24-q25. Cellular Component: dendrite cytoplasm; Golgi apparatus; neuron projection; focal adhesion; endoplasmic reticulum; integral to plasma membrane; plasma membrane; perikaryon; sarcolemma; lipid raft. Molecular Function: voltage-gated calcium channel activity; G-protein coupled receptor activity; neuropeptide binding; protein domain specific binding; protein binding; G-protein beta-subunit binding; G-protein alpha-subunit binding; beta-endorphin receptor activity; filamin binding. Biological Process: response to food; positive regulation of neurogenesis; positive regulation of nitric oxide biosynthetic process; wound healing; cellular response to stress; negative regulation of nitric oxide biosynthetic process; negative regulation of adenylate cyclase activity; locomotory behavior; response to lipopolysaccharide; behavioral response to ethanol; response to cocaine; sensory perception of pain; G-protein signaling, coupled to cyclic nucleotide second messenger; synaptic transmission; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; reduction of cytosolic calcium ion concentration; sensory perception; positive regulation of appetite; opioid receptor, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; dopamine receptor, adenylate cyclase activating pathway; acute inflammatory response to antigenic stimulus. Disease: Epilepsy, Idiopathic Generalized

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800