Collagen VII alpha1 Antibody | MyBioSource MBS9384295 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Collagen VII alpha1 Antibody

catalog :

MBS9384295

quantity :

0.1 mL

price :

440 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Collagen VII ?1 using HeLa whole cell lysates

product information

catalog number :

MBS9384295

products type :

Antibody

products full name :

Collagen VII alpha1 Antibody

products short name :

[Collagen VII alpha1]

other names :

[collagen alpha-1(VII) chain; Collagen alpha-1(VII) chain; collagen alpha-1(VII) chain; collagen type VII alpha 1 chain; Long-chain collagen; LC collagen]

other gene names :

[COL7A1; COL7A1; EBD1; EBR1; EBDCT; NDNC8; LC collagen]

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

2944

specificity :

Collagen VII alpha1 Ab detects endogenous levels of total Collagen VII alpha 1.

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

form :

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0mg/ml

storage stability :

Store at -20°C. Stable for 12 months from date of receipt

tested application :

Western Blot (WB)

app notes :

WB 1:1000-3000. For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight.

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human Collagen VII alpha 1. Subcellular location: Secreted > extracellular space > extracellular matrix >basement membrane.

ncbi gi num :

4502961

ncbi acc num :

NP_000085.1

ncbi gb acc num :

NM_000094.3

uniprot acc num :

Q02388

ncbi mol weight :

295 kDa

ncbi pathways :

Anchoring Fibril Formation Pathway (730307); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Extracellular Matrix Organization Pathway (576262); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

ncbi summary :

This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq, Jul 2008]

uniprot summary :

COL7A1: the alpha chain of type VII collagen, an extra-cellular basement membrane protein restricted to the zone beneath stratified squamous epithelia. Type VII collagen fibrils are composed of three identical alpha collagen chains. Forms anchoring fibrils, which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Dystrophic epidermolysis bullosa can result from defects in this protein or an autoimmune response made to type VII collagen. Homotrimer. Interacts with MIA3, facilitating its loading into transport carriers and subsequent secretion. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Extracellular matrix; Inhibitor; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: collagen type VII; endoplasmic reticulum lumen; ER to Golgi transport vesicle; ER-Golgi intermediate compartment membrane; extracellular matrix; extracellular region; extracellular space. Molecular Function: protein binding. Biological Process: collagen catabolic process; COPII coating of Golgi vesicle; epidermis development; ER to Golgi vesicle-mediated transport; extracellular matrix organization and biogenesis. Disease: Epidermolysis Bullosa Dystrophica, Autosomal Dominant; Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Epidermolysis Bullosa Dystrophica, Pretibial; Epidermolysis Bullosa Pruriginosa; Epidermolysis Bullosa With Congenital Localized Absence Of Skin And Deformity Of Nails; Nail Disorder, Nonsyndromic Congenital, 8; Transient Bullous Dermolysis Of The Newborn

size1 :

0.1 mL

price1 :

440 USD

size2 :

0.2 mL

price2 :

715

more info or order :

MyBioSource product webpage