AMPKB1 (Phospho-Ser108) Antibody | MyBioSource MBS9383512 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

AMPKB1 (Phospho-Ser108) Antibody

catalog :

MBS9383512

quantity :

0.1 mL

price :

440 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of AMPKB1 (Phospho-Ser108) using UV treated Jurkat whole cell lysates. -/+ means absence or presence of N peptide?non-phospho peptide) and P peptide(phospho peptide).

image 2 :

At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.

image 3 :

At 1/200 staining Mouse intestinal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.

product information

catalog number :

MBS9383512

products type :

Antibody

products full name :

AMPKB1 (Phospho-Ser108) Antibody

products short name :

[AMPKB1]

other names :

[5'-AMP-activated protein kinase subunit beta-1; 5'-AMP-activated protein kinase subunit beta-1; 5'-AMP-activated protein kinase subunit beta-1; protein kinase AMP-activated non-catalytic subunit beta 1]

products gene name :

[AMPKB1]

other gene names :

[PRKAB1; PRKAB1; AMPK; HAMPKb; AMPK; AMPK subunit beta-1; AMPKb]

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

270

specificity :

AMPKB1 (Phospho-Ser108) Ab detects endogenous levels of AMPKB1 only when phosphorylated at Ser108.

purity :

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

form :

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1.0mg/ml

storage stability :

Store at -20°C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB 1:1000-3000. IHC 1:50-1:200. IF/ICC 1:100-1:500. For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.

image5 heading :

Immunofluorescence (IF)

image5 description :

Staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary Ab was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary Ab.

other info1 :

Immunogen: A synthesized peptide derived from human AMPKB1 (Phospho-Ser108). Similarity: The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity. Belongs to the 5'-AMP-activated protein kinase beta subunit family.

ncbi gi num :

19923359

ncbi acc num :

NP_006244.2

ncbi gb acc num :

NM_006253.4

uniprot acc num :

Q9Y478

ncbi mol weight :

38 kDa

ncbi pathways :

AMPK Signaling Pathway (198868); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Circadian Rhythm Pathway (83084); Circadian Rhythm Pathway (495); Direct P53 Effectors Pathway (137939); Energy Metabolism Pathway (198907); Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway (160972); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591)

ncbi summary :

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]

uniprot summary :

AMPKB1: a non-catalytic subunit of AMPK, a conserved kinase of the CAMKL family. AMPK is an energy-sensing protein that plays a key role in regulating cellular energy homeostasis. Environmental stress, such as heat shock, nutrient deprivation, hypoxia and ischemia, indirectly activate AMPK by the depletion of cellular ATP and the concomitant rise of ADP and AMP levels. Allosteric activation is achieved primarily by rising ADP levels, and not solely by AMP levels as previously thought. Activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Beta subunits act as scaffolds on which the AMPK complex assembles, via its C-terminus that bridges alpha and gamma subunits. AMPK-beta1 or -beta2 subunits are required for assembling of AMPK heterotrimers and are important for regulating enzyme activity and cellular localization. AMPK beta1beta2 null mouse muscles reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise. Phosphorylation by ULK1 and ULK2 inhibits AMPK activity. Hematopoietic AMPKB1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity. Protein type: Autophagy; Protein kinase, regulatory subunit. Chromosomal Location of Human Ortholog: 12q24.23. Cellular Component: cytosol; nucleoplasm. Molecular Function: protein binding; protein kinase activity. Biological Process: cell cycle arrest; macroautophagy; protein amino acid phosphorylation

size1 :

0.1 mL

price1 :

440 USD

size2 :

0.2 mL

price2 :

715

more info or order :

MyBioSource product webpage