Phospho-VE-Cadherin (Tyr731) Antibody | MyBioSource MBS9382362 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-VE-Cadherin (Tyr731) Antibody

catalog :

MBS9382362

quantity :

0.1 mL

price :

440 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemistry analysis of paraffin-embedded human brain tissue using VE-Cadherin (Phospho-Tyr731) antibody.

image 2 :

Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3mM, 40mins), using VE-Cadherin (Phospho-Tyr731) antibody.

product information

catalog number :

MBS9382362

products type :

Antibody

products full name :

Phospho-VE-Cadherin (Tyr731) Antibody

products short name :

[VE-Cadherin]

products name syn :

[7B4 antigen; CADH5; Cadherin-5; CD144 antigen; Vascular endothelial-cadherin precursor; VE-cadherin]

other names :

[cadherin-5 preproprotein; Cadherin-5; cadherin-5; cadherin 5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144]

other gene names :

[CDH5; CDH5; 7B4; CD144; VE-cadherin]

clonality :

polyclonal

host :

rabbit

reactivity :

Human (Identities = 100%, Positives = 100%);. Mouse (Identities = 92%, Positives = 100%)

sequence length :

784

specificity :

VE-Cadherin (Phospho-Tyr731) antibody detects endogenous levels of VE-Cadherin only when phosphorylated at tyrosine 731.

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C/1 year

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500~1:3000. IHC: 1:50~1:100. ELISA: 1:20000

image1 heading :

Immunohistochemistry

image2 heading :

Western blot

other info1 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human VE-Cadherin around the phosphorylation site of tyrosine 731 (H-I-YP-G-Y). All Sites: Human: Tyr731; Mouse Tyr731

other info2 :

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

ncbi gi num :

166362713

ncbi acc num :

NP_001786.2

ncbi gb acc num :

NM_001795.4

uniprot acc num :

P33151

ncbi mol weight :

74,499 Da

ncbi pathways :

Adherens Junctions Interactions Pathway (119533); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); S1P2 Pathway (138059); Signaling Events Mediated By VEGFR1 And VEGFR2 Pathway (137940)

ncbi summary :

This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015]

uniprot summary :

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain. Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 16q21. Cellular Component: cell junction; cell surface; intercellular junction; membrane; plasma membrane. Molecular Function: beta-catenin binding; protein binding; receptor binding. Biological Process: intercellular junction assembly; transforming growth factor beta receptor signaling pathway

size1 :

0.1 mL

price1 :

440 USD

size2 :

0.2 mL

price2 :

715

more info or order :

MyBioSource product webpage