Phospho-PI3-kinase p85- alpha/ gamma (Tyr467/199) Antibody | MyBioSource MBS9382339 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-PI3-kinase p85- alpha/ gamma (Tyr467/199) Antibody

catalog :

MBS9382339

quantity :

0.1 mL

price :

440 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of PI3-kinase p85- alpha/ gamma phosphorylation expression in H2O2 treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

product information

catalog number :

MBS9382339

products type :

Antibody

products full name :

Phospho-PI3-kinase p85- alpha/ gamma (Tyr467/199) Antibody

products short name :

[PI3-kinase p85- alpha/ gamma]

products name syn :

[GRB1, P85A, PI3-kinase p85-alpha subunit, PI3K, PI3K p85-alpha, Phosphatidylinositol 3-kinase regulatory alpha subunit, PtdIns-3-kinase p85-alpha; P55G, PI3-kinase p85-gamma subunit, PI3K p85-gamma, Phosphatidylinositol 3-kinase regulatory gamma subunit, PtdIns-3-kinase p85-gamma, p55PIK]

other names :

[phosphatidylinositol 3-kinase regulatory subunit alpha isoform 4; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit 1; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha]

other gene names :

[PIK3R1; PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA; GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha]

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human, Mouse, Rat

sequence length :

361

specificity :

Phospho-PI3-kinase p85- alpha/ gamma (Tyr467/199) Antibody detects endogenous levels of PI3-kinase p85- alpha/ gamma only when phosphorylated at Tyrosine 467/199

purity :

Immunogen affinity purified

storage stability :

Store at -20 °C. Stable for 12 months from date of receipt

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

app notes :

WB 1:500-1:2000. IF/ICC 1:100-1:500. Optimal dilutions/concentrations should be determined by the end user.

image1 heading :

Western blot

other info1 :

Immunogen: A synthesized peptide derived from human PI3-kinase p85- alpha/ gamma around the phosphorylation site of Tyrosine 467/199.

other info2 :

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

products description :

PIK3R1 is a regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids.

ncbi gi num :

335057534

ncbi acc num :

NP_001229395.1

ncbi gb acc num :

NM_001242466.1

uniprot acc num :

P27986

ncbi mol weight :

54,83kDa

ncbi pathways :

3-phosphoinositide Biosynthesis Pathway (545314); 3-phosphoinositide Biosynthesis Pathway (138600); AMPK Signaling Pathway (198868); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Alpha6-Beta4 Integrin Signaling Pathway (198807); Amoebiasis Pathway (167324)

ncbi summary :

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

uniprot summary :

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).

size1 :

0.1 mL

price1 :

440 USD

size2 :

0.2 mL

price2 :

715

more info or order :

MyBioSource product webpage