product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Keratin, type II cytoskeletal 1b, KRT77 ELISA Kit
catalog :
MBS938019
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS938019
products type :
ELISA Kit
products full name :
Human Keratin, type II cytoskeletal 1b, KRT77 ELISA Kit
products short name :
keratin 77
products name syn :
Human Keratin; type II cytoskeletal 1b (KRT77) ELISA kit; K1B; KRT1B; MGC148087; keratin 1B; keratin 77
other names :
keratin, type II cytoskeletal 1b; Keratin, type II cytoskeletal 1b; Cytokeratin-1B; CK-1B; Keratin-77; K77; Type-II keratin Kb39
products gene name :
KRT77
other gene names :
KRT77; KRT1B; CK-1B; K77
uniprot entry name :
K2C1B_HUMAN
reactivity :
Human
sequence length :
578
specificity :
This assay has high sensitivity and excellent specificity for detection of human KRT77. No significant cross-reactivity or interference between human KRT77 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0. 156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human KRT77 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KRT77 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT77 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT77 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT77 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
194018511
ncbi acc num :
NP_778253.2
ncbi gb acc num :
NM_175078.2
uniprot acc num :
Q7Z794
ncbi mol weight :
61,901 Da
ncbi summary :
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13.[provided by RefSeq, Jun 2009]
uniprot summary :
K77: Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13.[provided by RefSeq, Jun 2009]. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 12q13.13. Cellular Component: cytoskeleton; keratin filament. Molecular Function: structural molecule activity
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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