Human Biglycan, BGN ELISA Kit | MyBioSource MBS937966 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Biglycan, BGN ELISA Kit

catalog :

MBS937966

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS937966

products type :

ELISA Kit

products full name :

Human Biglycan, BGN ELISA Kit

products short name :

biglycan

products name syn :

Human Biglycan (BGN) ELISA kit; DSPG1; PG-S1; PGI; SLRR1A; biglycan proteoglycan; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; small leucine-rich protein 1A; biglycan

other names :

biglycan preproprotein; Biglycan; biglycan; biglycan proteoglycan; bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; small leucine-rich protein 1A; dermatan sulphate proteoglycan I; biglycan; Bone/cartilage proteoglycan I; PG-S1

products gene name :

BGN

other gene names :

BGN; BGN; PGI; DSPG1; PG-S1; SLRR1A; SLRR1A

uniprot entry name :

PGS1_HUMAN

reactivity :

Human

sequence length :

368

specificity :

This assay has high sensitivity and excellent specificity for detection of Human BGN. No significant cross-reactivity or interference between Human BGN and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BGN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BGN bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4502403

ncbi acc num :

NP_001702.1

ncbi gb acc num :

NM_001711.4

uniprot acc num :

P21810

ncbi mol weight :

41,654 Da

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); CS/DS Degradation Pathway (645311); Chondroitin Sulfate Biosynthesis Pathway (645309); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Dermatan Sulfate Biosynthesis Pathway (645310); Glycosaminoglycan Metabolism Pathway (645297); Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway (645304); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196)

ncbi summary :

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex. [provided by RefSeq, Nov 2009]

uniprot summary :

BGN: May be involved in collagen fiber assembly. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: Xq28. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; lysosomal lumen; transport vesicle; cell surface; Golgi lumen; extracellular region; sarcolemma. Molecular Function: glycosaminoglycan binding; extracellular matrix binding; extracellular matrix structural constituent. Biological Process: chondroitin sulfate metabolic process; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; chondroitin sulfate catabolic process; blood vessel remodeling; pathogenesis; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; dermatan sulfate biosynthetic process

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage