Human Histone H3.1, HIST1H3A ELISA Kit | MyBioSource MBS937951 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Histone H3.1, HIST1H3A ELISA Kit

catalog :

MBS937951

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS937951

products type :

ELISA Kit

products full name :

Human Histone H3.1, HIST1H3A ELISA Kit

products short name :

histone cluster 1, H3a

products name syn :

Human Histone H3.1 (HIST1H3A) ELISA kit; H3/A; H3FA; H3 histone family; member A; histone 1; H3a; histone cluster 1; H3a

other names :

histone H3.1; Histone H3.1; histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A; histone cluster 1, H3a; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l

products gene name :

HIST1H3A

other gene names :

HIST1H3A; HIST1H3A; H3/A; H3FA; H3FA

uniprot entry name :

H31_HUMAN

reactivity :

Human

sequence length :

136

specificity :

This assay has high sensitivity and excellent specificity for detection of human HIST1H3A. No significant cross-reactivity or interference between human HIST1H3A and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of human HIST1H3A is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIST1H3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIST1H3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIST1H3A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIST1H3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4504281

ncbi acc num :

NP_003520.1

ncbi gb acc num :

NM_003529.2

uniprot acc num :

P68431

ncbi mol weight :

15,404 Da

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amyloids Pathway (366238); Disease Pathway (530764); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Gene Expression Pathway (105937); Hemostasis Pathway (106028); Meiosis Pathway (477133); Meiotic Recombination Pathway (205242); RNA Polymerase I Chain Elongation Pathway (106555)

ncbi summary :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

uniprot summary :

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 6p22.2. Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus. Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity. Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; DNA replication-dependent nucleosome assembly; DNA methylation on cytosine; regulation of gene expression, epigenetic

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage