Human Epoxide hydrolase 2, EPHX2 ELISA Kit | MyBioSource MBS937894 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Epoxide hydrolase 2, EPHX2 ELISA Kit

catalog :

MBS937894

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS937894

products type :

ELISA Kit

products full name :

Human Epoxide hydrolase 2, EPHX2 ELISA Kit

products short name :

epoxide hydrolase 2, cytoplasmic

products name syn :

Human Epoxide hydrolase 2 (EPHX2) ELISA kit; CEH; SEH; epoxide hydratase; epoxide hydrolase 2; cytosolic; epoxide hydrolase; soluble; epoxide hydrolase 2; cytoplasmic

other names :

bifunctional epoxide hydrolase 2 isoform b; Bifunctional epoxide hydrolase 2; bifunctional epoxide hydrolase 2; epoxide hydratase; epoxide hydrolase, soluble; epoxide hydrolase 2, cytosolic; epoxide hydrolase 2, cytoplasmic; Epoxide hydratase; Soluble epoxide hydrolase; SEH

products gene name :

EPHX2

other gene names :

EPHX2; EPHX2; CEH; SEH; CEH; SEH

uniprot entry name :

HYES_HUMAN

reactivity :

Human

sequence length :

502

specificity :

This assay has high sensitivity and excellent specificity for detection of Human EPHX2. No significant cross-reactivity or interference between Human EPHX2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml-1500 pg/ml. Sensitivity: 5.86 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 3: Aromatic hydrocarbons catabolism

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPHX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPHX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPHX2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPHX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

374532800

ncbi acc num :

NP_001243411.1

ncbi gb acc num :

NM_001256482.1

uniprot acc num :

P34913

ncbi mol weight :

62,616 Da

ncbi pathways :

Arachidonate Epoxygenase / Epoxide Hydrolase Pathway (198838); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Peroxisome Pathway (131226); Peroxisome Pathway (131126)

ncbi summary :

This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

uniprot summary :

EPHX2: Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate. Belongs to the AB hydrolase superfamily. Epoxide hydrolase family. Protein type: EC 3.1.3.76; Lipid Metabolism - arachidonic acid; EC 3.3.2.10; Hydrolase. Chromosomal Location of Human Ortholog: 8p21. Cellular Component: cytoplasm; peroxisome; cytosol. Molecular Function: toxin binding; protein homodimerization activity; lipid-phosphate phosphatase activity; epoxide hydrolase activity; phosphoric monoester hydrolase activity; magnesium ion binding; lipid phosphatase activity; receptor binding. Biological Process: cellular calcium ion homeostasis; cholesterol homeostasis; dephosphorylation; response to toxin; xenobiotic metabolic process; regulation of blood pressure; arachidonic acid metabolic process; epoxygenase P450 pathway; stilbene catabolic process; positive regulation of vasodilation; inflammatory response; drug metabolic process; phospholipid dephosphorylation. Disease: Hypercholesterolemia, Familial

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage