Human Adenylyl cyclase-associated protein 1, CAP1 ELISA Kit | MyBioSource MBS937628 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Adenylyl cyclase-associated protein 1, CAP1 ELISA Kit

catalog :

MBS937628

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS937628

products type :

ELISA Kit

products full name :

Human Adenylyl cyclase-associated protein 1, CAP1 ELISA Kit

products short name :

CAP, adenylate cyclase-associated protein 1 (yeast)

products name syn :

Human Adenylyl cyclase-associated protein 1 (CAP1) ELISA kit; RP11-115D7.1; CAP; CAP1-PEN; OTTHUMP00000004822; adenylyl cyclase-associated protein; CAP; adenylate cyclase-associated protein 1 (yeast)

other names :

adenylyl cyclase-associated protein 1; Adenylyl cyclase-associated protein 1; adenylyl cyclase-associated protein 1; CAP 1; CAP, adenylate cyclase-associated protein 1 (yeast)

products gene name :

CAP1

other gene names :

CAP1; CAP1; CAP; CAP1-PEN; CAP; CAP 1

uniprot entry name :

CAP1_HUMAN

reactivity :

Human

sequence length :

475

specificity :

This assay has high sensitivity and excellent specificity for detection of human CAP1. No significant cross-reactivity or interference between human CAP1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of human CAP1 is typically less than 6.25 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

157649073

ncbi acc num :

NP_001099000.1

ncbi gb acc num :

NM_001105530.1

uniprot acc num :

Q01518

ncbi mol weight :

51,901 Da

ncbi pathways :

Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Hemostasis Pathway (106028); Insulin Signaling Pathway (198845); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); Role Of Abl In Robo-Slit Signaling Pathway (119531); Signaling By Robo Receptor Pathway (119527)

ncbi summary :

The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2008]

uniprot summary :

CAP1: an actin binding protein. Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity. Protein type: Actin-binding; Cytoskeletal. Chromosomal Location of Human Ortholog: 1p34.2. Cellular Component: cortical actin cytoskeleton; focal adhesion; plasma membrane. Molecular Function: actin binding. Biological Process: receptor-mediated endocytosis; platelet activation; axon guidance; platelet degranulation; ameboidal cell migration; adenylate cyclase activation; establishment and/or maintenance of cell polarity; signal transduction; actin cytoskeleton organization and biogenesis; blood coagulation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage