product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Chitotriosidase-1, CHIT1 ELISA Kit
catalog :
MBS937536
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS937536
products type :
ELISA Kit
products full name :
Mouse Chitotriosidase-1, CHIT1 ELISA Kit
products short name :
chitinase 1 (chitotriosidase)
products name syn :
Mouse Chitotriosidase-1 (CHIT1) ELISA kit; CHI3; CHIT; FLJ00314; MGC125322; chitotriosidase; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase; chitinase 1 (chitotriosidase)
other names :
chitotriosidase-1 isoform 2; Chitotriosidase-1; chitotriosidase-1; chitinase-1; chitinase 1 (chitotriosidase); Chitinase-1
products gene name :
CHIT1
other gene names :
Chit1; Chit1; 2300002L19Rik
uniprot entry name :
CHIT1_MOUSE
reactivity :
Mouse
sequence length :
396
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse CHIT1. No significant cross-reactivity or interference between Mouse CHIT1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: glyconeogenesis and glycometabolism. Protein Biological Process 3: Carbohydrate metabolism
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHIT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHIT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHIT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHIT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
548961918
ncbi acc num :
NP_001271453.1
ncbi gb acc num :
NM_001284524.1
uniprot acc num :
Q9D7Q1
ncbi mol weight :
51,112 Da
ncbi pathways :
Amino Sugar And Nucleotide Sugar Metabolism Pathway (83178); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350)
uniprot summary :
CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 3.2.1.14; Hydrolase. Cellular Component: extracellular space; lysosome; extracellular region. Molecular Function: endochitinase activity; hydrolase activity; hydrolase activity, acting on glycosyl bonds; chitin binding; chitinase activity; hydrolase activity, hydrolyzing O-glycosyl compounds. Biological Process: polysaccharide catabolic process; metabolic process; carbohydrate metabolic process; immune response; chitin catabolic process; chitin metabolic process
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

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