catalog number :
MBS937484
products type :
ELISA Kit
products full name :
Chicken Platelet-activating factor acetylhydrolase, PLA2G7 ELISA Kit
products short name :
phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
products name syn :
Chicken Platelet-activating factor acetylhydrolase (PLA2G7) ELISA kit; LDL-PLA2; LP-PLA2; PAFAH; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; PAF 2-acylhydrolase; lipoprotein-associated phospholipase A2; phospholipase A2; ; phospholipase A2; group VII (platelet-activating factor acetylhydrolase; plasma)
other names :
platelet-activating factor acetylhydrolase; Platelet-activating factor acetylhydrolase; platelet-activating factor acetylhydrolase; LDL-PLA(2); PAF 2-acylhydrolase; PAF acetylhydrolase; LDL-associated phospholipase A2; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; LDL-associated phospholipase A2; LDL-PLA(2); PAF 2-acylhydrolase
products gene name :
PLA2G7
other gene names :
PLA2G7; PLA2G7; PAF acetylhydrolase; LDL-PLA(2)
uniprot entry name :
PAFA_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of chicken PLA2G7. No significant cross-reactivity or interference between chicken PLA2G7 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 18.8 pg/ml-1200 pg/ml. Sensitivity: The minimum detectable dose of chicken PLA2G7 is typically less than 9.4 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest chicken PLA2G7 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PLA2G7. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for PLA2G7. The competitive inhibition reaction is launched between with pre-coated PLA2G7 and PLA2G7 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of PLA2G7 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_990300.1
ncbi gb acc num :
NM_204969.1
ncbi mol weight :
47,046 Da
ncbi pathways :
Ether Lipid Metabolism Pathway (83901); Ether Lipid Metabolism Pathway (365)
uniprot summary :
Function: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. Catalytic activity: 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
size4 :
10x96-Strip-Wells